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Introduction Cervical cancer is one of the leading causes of mortality among women population worldwide. In spite of recurrent screening, vaccination, and chemotherapeutic interventions, combating cervical cancer still remains a challenge. Crizotinib is a small molecule inhibitor that targets mesenchymal epithelial transition factor (c‐MET) and has been successfully studied for its anti‐cancer effects in non‐small cell lung cancer, pancreatic, gastric, renal, prostate, and breast carcinomas. Although c‐MET is a well‐known prognostic, diagnostic, and therapeutic target in cervical cancer, anti‐cancer properties of its inhibitor crizotinib against cervical carcinoma, has not been explored yet. Methods In the present study, the anti‐cancer effects of crizotinib on cervical cancer cells were evaluated using various in vitro cell‐based assays, such as labelling drug‐treated cells with MTT, H2DCFDA, Annexin V5‐fluorescein isothiocyanate (FITC) antibody, JC‐1, PI, and analysis using fluorescence‐activated cell sorting (FACS). Results The molecule was found to effectively inhibit proliferation of cervical cancer cells HeLa and SiHa with an IC50 of 0.641 ± 0.0724 and 0.871 ± 0.104 μM, respectively, and induce apoptosis in a dose‐dependent manner. Further investigations showed that crizotinib‐induced production of reactive oxygen species (ROS) with increasing concentrations further resulted in mitochondrial membrane depolarization. However, the drug had no effect on cell cycle progression of HeLa and SiHa cells. Conclusion Thus, the study elucidates the cytotoxic effects of crizotinib in cervical cancer cells by activation of ROS‐dependent apoptotic pathway via mitochondrial depolarization. These findings will further aid the evaluation of other molecular mechanisms of crizotinib and would pave the way for its implication as a chemotherapeutic option in cervical cancer.

In the present study, prominently evolved varieties of Indica rice (Oryza sativa L.) were studied and characterized. Effect of temperature and humidity on the grain yield and grain quality in different geographical areas of Northern India was studied. This would ensure the selection of rice varieties with respect to different geographical climatic regions. Seven varieties of rice were studied and characterized by the effect on their morpho-physiochemical characters in different climatic conditions of Uttar Pradesh and Haryana, India. All the seven varieties of rice except Sambha Sub-1 showed presence of fgr gene whose protein product are responsible for the peculiar fragrance of Basmati rice. This was also confirmed by the chemical assay for aroma. Morho-physiological characters of the crop namely; plant height, plant biomass, root biomass, leaf area, panicle length, spikelets per panicle, grain yield per plant and days of maturity were found to significantly correlate with each other with (P > 0.5). Biochemical studies performed showed the effect of temperature in different climatic conditions on total chlorophyll, chlorophyll a and chlorophyll b, total sugar and total protein. A significant correlation (p > 0.05) was observed between the higher and lower temperature and humidity respectively and different morpho-physiochemical characters. Four varieties of Basmati namely VB-21, VB-24, PB-1509, PB-1121 has been found to show higher morphophysiochemical characters and grain yield per plant. VB-21 and VB-24 has been observed to resist the high temperature and low humidity in the region of Haryana as they have shown to produce higher grain yield, seed weight and grain quality. …………………………………………………………………………………………………….... Introduction:-Rice is one of the most important field crops of the world providing staple food to millions. It is grown in 114 countries across the world and the population of 150 million has continuing cultivation in near 11% of the world's cultivated land. More than 90% of the world rice is produced and consumed in Asia as it is an indispensable source of calories for almost half of the world population within the continent. Rice belongs to the genus Oryza and has two cultivated and 22 wild species. The two cultivated species are Oryza.sativa L and Oryza.glaberrima. Oryza.sativa L