This study evaluated the microbiological quality of milk and Coalho cheese, the prevalence of enterotoxin genes, antimicrobial resistance and determined an inducible MLSB resistance phenotype by the D-test in strains of Staphylococcus aureus isolated from these products. Seventy samples of milk and Coalho cheese were analyzed. S. aureus strains were identified by biochemical tests. The presence of se genes (sea-see) was tested by polymerase chain reaction. The antimicrobial sensitivity of S. aureus strains was evaluated for 13 antimicrobial drugs using the disk diffusion technique and the double-disk diffusion test (D-test) was performed to determine inducible resistance to lincosamide phenotype. The amount of toxin sufficient to cause foodborne diseases is generally observed when Staphylococcus populations exceed 105 CFU mL-1 g-1. In this study, none of the milk samples analyzed showed these counts; however, 73.3% (22/30) of Coalho cheese samples exceeded this value. A total of 109 isolates were identified as S. aureus. The presence of enterotoxin genes was detected in 25.7% of these isolates and amplified only for the sec gene. Most of the isolates (78.5%) were resistant to one or more antimicrobial agents. The D test showed that 25.0% of erythromycin-resistant isolates had the constitutive resistance phenotype, and 3.8% had the inducible resistance phenotype to clindamycin. These results indicate that these dairy products represent a health risk since these bacteria can cause foodborne diseases or may be a possible route for the transfer of antimicrobial resistance to humans.

No abstract

Background: Cowpea is a very popular crop in Brazil, rich in nutrients that can be used as food to feed the population, avoiding deficiencies caused by the lack of minerals such as iron and zinc. Objective: To select elite cowpea lines for biofortification of iron and zinc and determine the physical and chemical characteristics of the lines. Were analyzed: 33 cowpea genotypes, being 31 elite genotypes and two biofortified cultivars (control). Method: The 10 best lines with iron and zinc contents were selected and, together with the controls, were analyzed for chemical composition (moisture, ash, lipids, proteins and carbohydrates), total energy value and cooking time. Results: The iron and zinc contents in semi-prostrate genotypes were higher than those in semi-erect genotypes, all the semi-prostrate lines presented a high iron content, and 19.25% presented a high zinc content. The genotypes of the semi-erect lines exhibited more significant genetic variability for iron content, whereas those of the semi-prostrate genotypes showed more significant genetic variation for zinc content, both exhibited a high genetic component in phenotypic expression. Improving the genotypes for increased protein content led to reductions in lipid and carbohydrate contents, as well as the total energy value, whereas increasing the carbohydrate content increased both the total energy value and the zinc content. The MNC04-792F-146 lines presented alleles that favored an increase in carbohydrate content and total energy, while the MNC04-769F-26, MNC04-769F-31, and MNC04-774F-90 lines were shown to be good sources of genes for increasing protein content, decreasing lipid content, and rapid cooking. Conclusion: The MNC04-762F-9, MNC04-792F-146, and MNC04-769F-55 lines exhibited the greatest potential to be released as iron and zinc biofortified cultivars.

No abstract

O leite é um alimento consumido por grande parte da população. Embora perecível, a aplicação de tratamento térmico, como o UAT (Ultra Alta Temperatura), estende a sua conservação, variando de três a seis meses. Neste estudo avaliou-se a qualidade do leite integral UAT comercializado em supermercados de Teresina, PI. Os parâmetros físicoquímicos verificados foram densidade, gordura, proteína, extrato seco desengordurado, crioscopia e condutividade, determinadas por método rápido utilizando o aparelho de ultrassom Ekomilk®, e também acidez e pH, segundo metodologia oficial. As análises mostraram que 45% das marcas de leite integrais UAT estavam dentro dos padrões oficiais. Em relação aos parâmetros de pH, densidade e proteína, os resultados