Dye photobleaching is a photochemical reaction that can be investigated locally by fluorescence microscopy techniques. In this study a user-friendly computational tool to assist photobleaching experiments called Photobleaching Lifetime Imaging Microscopy (PbLIM) is presented. With this tool it is possible to recover the photobleaching kinetics spatially, where a photobleaching lifetime is generated for each pixel of the image. Our model was applied to the photobleaching process of thionine encapsulated into the one-dimensional nano channels of Zeolite L (ZL), from where we gained insight on the molecular oxygen distribution inside ZL channels, as well as on the detailed photobleaching of the confined thionine.
The combination of a sensitizer and TiO nanoparticles forming a photocatalytic material is a central issue in many fields of applied photochemistry. The charge injection of emissive sensitizers into the conduction band of the semiconductor TiO may form a photoactive region that becomes dark, or it has a very low emission signal due to the generation of sensitizer radicals. However, by sequential coupling of a selected photoredox dye, such as resazurin, the dark region may become fluorescent at the interfaces where the charge injection has taken place due to the concomitant formation of fluorescent resorufin by cascade electron transfer. Using this strategy and a total internal reflection fluorescence microscopy (TIRFM) image, the charge injection in TiO/CdS and SiO/TiO/CdS nanoparticles is investigated The method allows the charge injection efficiency of the excited CdS into TiO to be evaluated qualitatively, explaining the differences observed for these photocatalytic materials in H generation.
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