We demonstrate the trapping of elastic particles by the large gradient force of a single acoustical beam in three dimensions. Acoustical tweezers can push, pull and accurately control both the position and the forces exerted on a unique particle. Forces in excess of 1 micronewton were exerted on polystyrene beads in the sub-millimeter range. A beam intensity less than 50 Watts/cm 2 was required ensuring damage-free trapping conditions. The large spectrum of frequencies covered by coherent ultrasonic sources provide a wide variety of manipulation possibilities from macroto microscopic length scales. Our observations could open the way to important applications, in particular in biology and biophysics at the cellular scale and for the design of acoustical machines in microfluidic environments.

This work aims to model the acoustic radiation forces acting on an elastic sphere placed in an inviscid fluid. An expression of the axial and transverse forces exerted on the sphere is derived. The analysis is based on the scattering of an arbitrary acoustic field expanded in the spherical coordinate system centered on the spherical scatterer. The sphere is allowed to be arbitrarily located. The special case of high order Bessel beams, acoustical vortices, are considered. These types of beams have a helicoidal wave front, i.e., a screw-type phase singularity and hence, the beam has a central dark core of zero amplitude surrounded by an intense ring. Depending on the sphere's radius, different radial equilibrium positions may exist and the sphere can be set in rotation around the beam axis by an azimuthal force. This confirms the pseudo-angular moment transfer from the beam to the sphere. Cases where the axial force is directed opposite to the direction of the beam propagation are investigated and the potential use of Bessel beams as tractor beams is demonstrated. Numerical results provide an impetus for further designing acoustical tweezers for potential applications in particle entrapment and remote controlled manipulation.

We present, in our knowledge, the first theoretical demonstration of the possibility to trap and manipulate particles in three dimensions with the radiation pressure exerted by a single acoustical beam. Numerical examples demonstrate that single-beam acoustical tweezers operating in three dimensions are feasible with a large variety of materials and may widely extend the range of forces and operation regions that are currently available with optical tweezers. To do so, a method to model the focusing properties of acoustical beams with complex wavefronts using a spherical transducer is proposed. Then, the radiation forces exerted by various beams going from the classical vortex to the high radial degree spherical vortex beam that we introduce here are studied. While the first is shown to trap moderately small particles, the latter will stiffly trap large solid spheres in three dimensions. Even though this demonstration is carried out using a formalism suited to acoustics, it is easily applicable to trap non-transparent particles with optical tweezers that remain an issue.

Contactless manipulation of microparticles using acoustic waves holds promise for applications ranging from cell sorting to three-dimensional (3D) printing and tissue engineering. However, the unique potential of acoustic trapping to be applied in biomedical settings remains largely untapped. In particular, the main advantage of acoustic trapping over optical trapping, namely the ability of sound to propagate through thick and opaque media, has not yet been exploited in full. Here we demonstrate experimentally the use of the recently developed technique of single-beam acoustical tweezers to trap microbubbles, an important class of biomedically relevant microparticles. We show that the region of vanishing pressure of a propagating vortex beam can confine a microbubble by forcing low-amplitude, nonspherical, shape oscillations, enabling its full 3D positioning. Our interpretation is validated by the absolute calibration of the acoustic trapping force and the direct spatial mapping of isolated bubble echos, for which both find excellent agreement with our theoretical model. Furthermore, we prove the stability of the trap through centimeter-thick layers of bio-mimicking, elastic materials. Finally, we demonstrate the simultaneous trapping of nanoparticle-loaded microbubbles and activation with an independent acoustic field to trigger the release of the nanoparticles. Overall, using exclusively acoustic powering to position and actuate microbubbles paves the way toward controlled delivery of drug payloads in confined, hard-to-reach locations, with potential in vivo applications.

The controlled rotation of solid particles trapped in a liquid by an ultrasonic vortex beam is observed. Single polystyrene beads, or clusters, can be trapped against gravity while simultaneously rotated. The induced rotation of a single particle is compared to a torque balance model accounting for the acoustic response of the particle. The measured torque (∼10  pN m for a driving acoustic power ∼40  W/cm^{2}) suggests two dominating dissipation mechanisms of the acoustic orbital angular momentum responsible for the observed rotation. The first takes place in the bulk of the absorbing particle, while the second arises as dissipation in the viscous boundary layer in the surrounding fluid. Importantly, the dissipation processes affect both the dipolar and quadrupolar particle vibration modes suggesting that the restriction to the well-known Rayleigh scattering regime is invalid to model the total torque even for spheres much smaller than the sound wavelength. The findings show that a precise knowledge of the probe elastic absorption properties is crucial to perform rheological measurements with maneuverable trapped spheres in viscous liquids. Further results suggest that the external rotational steady flow must be included in the balance and can play an important role in other liquids.

Biomedical microbubbles stabilized by a coating of magnetic or drug-containing nanoparticles show great potential for theranostics applications. Nanoparticle-coated microbubbles can be made to be stable, echogenic, and to release the cargo of drugcontaining nanoparticles with an ultrasound trigger. This article reviews the design principles of nanoparticle-coated microbubbles for ultrasound imaging and drug delivery, with a particular focus on the physical chemistry of nanoparticle-coated interfaces; the formation, stability and dynamics of nanoparticle-coated bubbles; and the conditions for controlled nanoparticle release in ultrasound. The emerging understanding of the modes of nanoparticle expulsion and of the transport of expelled material by microbubble-induced flow is paving the way towards more efficient nanoparticlemediated drug delivery. The article highlights the knowledge gap that still remains to be addressed before we can control these phenomena.

Studies on radiation pressure in acoustics and optics have enriched one another and have a long common history. Acoustic radiation pressure is used for metrology, levitation, particle trapping and actuation. However, the dexterity and selectivity of single-beam optical tweezers are still to be matched with acoustical devices. Optical tweezers can trap, move and positioned micron size particles, biological samples or even atoms with subnanometer accuracy in three dimensions. One limitation of optical tweezers is the weak force that can be applied without thermal damage due to optical absorption. Acoustical tweezers overcome this limitation since the radiation pressure scales as the field intensity divided by the speed of propagation of the wave. However, the feasibility of single beam acoustical tweezers was demonstrated only recently. In this paper, we propose a historical review of the strong similarities but also the specificities of acoustical and optical radiation pressures, from the expression of the force to the development of single-beam acoustical tweezers.

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