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Abstract-Cognitive Radio (CR) emerges as a promising solution to current unbalanced spectrum utilization. The cognitive ad hoc network can take advantage of dynamic spectrum access and spectrum diversity over wide spectrum. It could achieve higher network capacity compared to traditional ad hoc networks, thus supporting bandwidth-demanding applications. A cognitive radio operates over wide spectrum with unpredictable channel availability. Moreover, the transmission opportunity of a cognitive node is not guaranteed due to the presence of primary users (PUs). These two unique features define new routing problems in cognitive ad hoc networks. To better characterize the unique features of cognitive radio networks, we propose new routing metrics, including accumulated spectrum temperature, highest spectrum temperature, and mixed spectrum temperature to account for the timevarying spectrum availability. The proposed metrics favor the "coolest" path, or the path with the most balanced and/or the lowest spectrum utilization by the primary users. We also study the computational complexity of the routing algorithm in cognitive ad hoc networks. Experiment results on our USRP-2 testbed show that the proposed metrics are capable of capturing the fluctuation of spectrum availability and suitable for cognitive ad hoc networks.

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BackgroundThe alcohol dehydrogenase (ADH) system plays a critical role in sugar metabolism involving in not only ethanol formation and consumption but also the general “cofactor balance” mechanism. Candida maltosa is able to ferment glucose as well as xylose to produce a significant amount of ethanol. Here we report the ADH system in C. maltosa composed of three microbial group I ADH genes (CmADH1, CmADH2A and CmADH2B), mainly focusing on its metabolic regulation and physiological function.Methodology/Principal FindingsGenetic analysis indicated that CmADH2A and CmADH2B tandemly located on the chromosome could be derived from tandem gene duplication. In vitro characterization of enzymatic properties revealed that all the three CmADHs had broad substrate specificities. Homo- and heterotetramers of CmADH1 and CmADH2A were demonstrated by zymogram analysis, and their expression profiles and physiological functions were different with respect to carbon sources and growth phases. Fermentation studies of ADH2A-deficient mutant showed that CmADH2A was directly related to NAD regeneration during xylose metabolism since CmADH2A deficiency resulted in a significant accumulation of glycerol.Conclusions/SignificanceOur results revealed that CmADH1 was responsible for ethanol formation during glucose metabolism, whereas CmADH2A was glucose-repressed and functioned to convert the accumulated ethanol to acetaldehyde. To our knowledge, this is the first demonstration of function separation and glucose repression of ADH genes in xylose-fermenting yeasts. On the other hand, CmADH1 and CmADH2A were both involved in ethanol formation with NAD regeneration to maintain NADH/NAD ratio in favor of producing xylitol from xylose. In contrast, CmADH2B was expressed at a much lower level than the other two CmADH genes, and its function is to be further confirmed.

The interactions of hydrophobically modified poly(acrylamide)s (HMPAM) and unmodified poly-(acrylamide) (PAM) with sodium dodecyl sulfate (SDS) have been studied by electron spin resonance using 2,2,6,6-tetramethylpiperidine-1-oxyl and 4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl as spin probes. The morphologies of dried solutions of the polymers with and without SDS have also been observed by transmission electron microscopy. The rotational correlation time τc of the spin probes reflects the microenvironment of the polymer-micelle aggregates, which indicates that the higher hydrophobicity of HMPAMs leads to a much more compact packing in the polymer-micelle aggregates. The interactions between surfactants and polymers cause morphological transitions of polymers, which results in the polymer chains stretching out.