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Bacillus subtilis 430A, isolated from the Vernonia herbacea (Veil Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (K,,,, 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50°C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.

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