The aim of the present study was to evaluate the concentration of sodium pyruvate in growth media by using the mostprobable number method to decompose carried-over liquid hydrogen peroxide (H 2 O 2 ) without negative influences on correct enumeration of Geobacillus stearothermophilus spores. An equivalent molar ratio of sodium pyruvate and H 2 O 2 was verified to be sufficient for a complete decomposition. The results showed that by exposition of G. stearothermophilus spores in different liquid hydrogen peroxide and by carry-over of concentrations ranging from 3.56 Á 10 -6 up to 1.69 Á 10 -4 mol, a sodium pyruvate concentration of 0.05 mol L -1 in growth media was most efficient for the recovery of spores.
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