For the determination of accurate quantity of impurities in the samples authentic impurity standards or response factors at a given wavelength must be known. In the presented work a convenient method for determination of relative response factors of impurities has been described without using an authentic impurities standard. An approach for the determination of response factors of the impurities where impurity standard is physically not available was developed and verified using different approaches. One such method was developed and verified by RP-HPLC using Cosmosil C18 MS-II column at UV 238 nm. Two different approaches were employed and the verification of correctness of approach was done using a known related substance of known response factor.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.