The main objective of this research is to investigate the anti-biofilm and anti-bacterial activity of Sesbania grandiflora (S. grandiflora) against Staphylococcus aureus. S. grandiflora extract were prepared and analyzed with UV –Vis spectroscopy, Fourier transform infrared spectroscopy, Dynamic light scattering. Biofilm forming pathogens were identified by congo-red assay. Quantification of Extracellular polymeric substance (EPS) particularly protein and carbohydrate were calculated. The efficacy of the herbal extract S. grandiflora and its inhibition against the pathogenic strain of S. aureus was also evaluated. The gradual decrease or disappearance of peaks reveals the reduction of protein and carbohydrate content in the EPS of S. aureus when treated with S. grandiflora. The antibacterial activity of S. grandiflora extract against the bacterial strain S. aureus showed that the extract were more active against the strain. To conclude, anti-biofilm and antibacterial efficacy of S. grandiflora plays a vital role over biofilm producing pathogens and act as a good source for controlling the microbial population.
The medicative plants were copiously applied in the healing of health complications. Traditionally implemented, medicative-worthy Acacia nilotica (Fabaceae family), is applied globe-wide in customary healing practices. Here, we have tried to unravel the antioxidant, fungicidal, antibacterial and cytotoxic trait of A. nilotica bark aqueous extract. Phyto-content testing revealed presence of flavonoids, tannins, alkaloids, phenols, carbohydrates, proteins, and amino acids. The antioxidant efficacies were 39.12 µg/ml (for DPPH assay) and 42.64 µg/ml (for NO assay) as IC50 ranges, respectively. The bark aqueous extract manifested fungicidal impact on all the screened fungal strains with the highest inhibitory zone against A. fumigatus (19.5 ± 0.45 mm). The MTT assay of bark aqueous extract registered a dose-related cytotoxic impression with 39.05 µg/ml (IC50 outcome). Hence, it emphasizes strongly that A. nilotica bark aqueous extract is a prospective therapeutic source and it is indispensable to further scrutinize its therapeutic and curative properties.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.