Clean and efficient exfoliation of bulk MoS2 into single- or few-layered nanosheets in the pure semiconducting hexagonal phase (2H phase) remains a great challenge and becomes a bottleneck for the intensive studies and applications of MoS2-based nanomaterials. Here, a new method for the scalable synthesis of 2H-MoS2 nanosheets using liquid nitrogen (L-N2) exfoliation has been developed. After five heating/L-N2 immersion cycles, the produced MoS2 nanosheets are primarily fewer than three layers in the pure 2H phase after the evaporation of the exfoliation reagent of L-N2. Impressively, two-dimensional (2D) van der Waals heterostructures by accommodating organic semiconductive nanoaggregates on the surface of semiconducting 2H-MoS2 nanosheets exhibit excellent electronic rectification effects for a nonvolatile write-once-read-many-times memory behavior with an ON/OFF ratio of over 105. This work with the novel heating/L-N2 exfoliation strategy to prepare clean and pure 2H-MoS2 nanosheets would open up tremendous potential opportunities for the fundamental studies and practical applications of semiconducting 2D nanomaterials.

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TOB1, a member of the BTG/TOB protein family, inhibits tumor cell proliferation. We previously observed down-regulation and phosphorylation of TOB1 in gastric cancer (GC). Here, we examined the subcellular distribution and clinical significance of TOB1 expression and phosphorylation in GC. Immunohistochemical analysis of 341 primary GC and corresponding normal gastric tissue samples demonstrated that nuclear TOB1 expression was lower in GC than normal tissue (80.4% vs. 92.4%), and decreased nuclear TOB1 expression correlated with high TNM stage. By contrast, phosphorylation of nuclear TOB1 was higher in GC than normal gastric tissue (66.0% vs. 36.4%), and was associated with poorly differentiated and high TNM stage tumors. Patients with intestinal type GC and increased nuclear TOB1 phosphorylation had poor overall survival. Multivariate survival analysis indicated the nuclear concentration of phosphorylated TOB1 was an independent prognostic factor for intestinal type GC. Overexpression of TOB1 containing mutations in its nuclear export signal inhibited GC cell proliferation, migration, and invasion compared to cells expressing TOB1 with the nuclear localization signal. Thus, decreased TOB1 expression and increased phosphorylation of nuclear TOB1 is associated with aggressive tumor behavior and poor prognosis in intestinal type GC. Additionally, TOB1 nuclear retention is critical for its anti-proliferative activity.

By allelotyping for loss of heterozygosity (LOH), we previously identified a deletion region that harbors the candidate tumor suppressor gene DAL-1 at 18p11.3 in sporadic gastric cancers (GCs). The expression and function of DAL-1 in GCs remained unclear. Here, we demonstrated that the absence of or notable decreases in the expression of DAL-1 mRNA and protein was highly correlated with CpG hypermethylation of the DAL-1 promoter in primary GC tissues and in GC cell lines. Furthermore, abnormal DAL-1 subcellular localization was also observed in GC cells. Exogenous DAL-1 effectively inhibited cancer cell proliferation, migration, invasion and epithelial to mesenchymal transition (EMT); exogenous DAL-1 also promoted apoptosis in GC AGS cells. When endogenous DAL-1 was knocked down in GC HGC-27 cells, the cells appeared highly aggressive. Taken together, these findings provide solid evidence that aberrant expression of DAL-1 by hypermethylation in the promoter region results in tumor suppressor gene behavior that plays important roles in the malignancy of GCs. Understanding the role of it played in the molecular pathogenesis of GC, DAL-1 might be a potential biomarker for molecular diagnosis and evaluation of the GC.

In order to improve the sensitivity of liquid dielectric constant measurements, a liquid dielectric constant sensor based on a cubic container structure is proposed for the first time. The cubic container, which consists of a dielectric substrate with a split resonant ring (SRR) and microstrip lines, can enhance the electric field intensity in the measuring area. High sensitivity can be obtained from measuring the dielectric constant with the characteristics of the structure resonate. The research results show that the resonant frequency of the sensor is shifted from 7.69 GHz to 5.70 GHz, with about a 2 GHz frequency offset, when the dielectric constant of the sample varied from 1 to 10. A resonance frequency offset of 200 MHz for the per unit dielectric constant is achieved, which is excellent regarding performance. The permittivity of oil with a different metal content is measured by using the relation between the fitted permittivity and the resonant frequency. The relative error is less than 1.5% and the sensitivity of measuring is up to 3.45%.