Amylase is a hydrolytic enzyme that is used widely in industries. It is easier to produce enzymes from bacteria than any other organism. The present work focused on the production of amylase enzyme from bacteria isolated, from hot water spring of Manikaran. The amylase producing bacterial strain screening was performed using starch agar plates. Various biochemical tests were carried out and molecularly; it was identified as Bacillus cereus. Optimum conditions such as temperature, pH and incubation period were optimized for maximum enzyme production from B. cereus. The optimum pH was found to be 9.0 and temperature was 45°C and 72 h of incubation period. The enzyme was partially purified by ammonium sulfate precipitation method and it was purified by dialysis. After dialysis, partial purified enzyme was immobilized by entrapment method with sodium alginate as natural matrix. The highest operational stability was six reuses with 54% relative activity and storage stability up to 35 days with more than 50% residual activity. The K m and V max of immobilized enzyme were determined and compared with free enzyme.
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