Abstract. Lonthor DW, Miftahudin, Kayat, Julzarika A, Subehi L, Iswandono E, Dima AOM, Dianto A, Setiawan F, Nugraha MFI. 2023. Diversity of aquatic plants in the Rote Dead Sea area, East Nusa Tenggara, Indonesia, based on rbcL marker. Biodiversitas 24: 810-818. The Rote Dead Sea area in Rote Islands has two freshwater and 24 saltwater lakes. The saltwater lakes have salinity levels varied between 0-100 ppt. Every lake has specific aquatic plant species. Species identification through morphological analysis only has its limitations; therefore, DNA barcoding is a preference due to an effective, accurate, and faster method for species identification. In this study, we used the rbcL marker to identify and analyze the diversity of the aquatic plant species in lakes Ledulu, Oenduy, Oemasapoka, Oesotimori, and Landu Leko. Plants samples' DNA was amplified using rbcL primers, and the amplicons were sequenced. Species identification was based on morphological study and the rbcL sequence database. Diversity analyses were performed based on pairwise genetic distances, analysis of molecular variance, and genetic relationships based on the Kimura 2 parameter. The results showed that all 20 aquatic plants were correctly identified, and high genetic diversity was discovered. AMOVA analysis showed that genetic variations within the population were higher than among the population. All plant species were grouped into four groups based on the phylogenetic tree and fractional component analyses. The species belonging to the certain family were grouped in the same clade and the closely related plant families. The rbcL marker can be used to discriminate aquatic plant species and analyze their genetic diversity and relationships.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.