Ammonia analysis based on complexing ammonia with Cu2+ ions by UV-Vis spectrophotometry. This research aims to obtain uptake at the maximum wavelength of the copper-ammonia complex, optimum conditions and validation of UV-Vis spectrophotometry. The reaction between Cu2+ ions which are blue with ammonia colorless occur in solution to form complex compounds [Cu(NH3)4]2+ dark blue, the maximum absorbance is obtained at a wavelength of 615 nm. Optimization of the ammonia complexing the Cu2+ ions provide optimum conditions a solution of Cu2+ concentrations of 0.01 M in ammonia concentration of 0.04 M, pH 7, and the optimum time to form a complex occurred in the 30th minute and complex compounds can be stable for 90 minutes (± 1 hours 30 minutes). The analytical method validation ammonia using Cu2+ ions provide linear regression equation y = 4,772 x + 0.333 with R2 = 0.989 ammonia concentration range of 0.003 M to 0.08 M; LOD 0.01 M; LOQ 0.04 M; % RSD = 1.32%; and% recovery = 102.03%. The concentration of ammonia in the wastewater sample application obtained the ammonia concentration of 0.04 M. Based on the results of the validation, Ammonia solution with Cu2+ ion as a complex used for the determination of ammonia levels by UV-Vis spectrophotometry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.