\s=b\The distribution and number of lymphocytes, monocytes/macrophages, and cells expressing HLA-DR antigen were studied in frozen biopsy sections of nasal mucosa from 40 healthy adults, using monoclonal antibody avidin-biotin immunoperoxidase techniques. The lymphocyte to monocyte/macrophage ratio was estimated to be 10:1; the T cell to B cell ratio was 3:1; and the T helper/inducer cell to T suppressor/cytotoxic cell ratio averaged 2.5:1. Regional differences were observed with a relatively increased number of T suppressor /cytotoxic cells around submucosal glands, and a relatively large number of B cells in lymphocyte aggregates in the lamina propria. The HLA-DR antigen was expressed in epithelial cells, suggesting involvement of surface epithelium of human airway in local immune responses. (Arch Otolaryngol Head Neck Surg 1987;113:59-62) Lymphocytes are a normal cell pop-J ulation in the airway mucosa from the nose to the alveoli12; howev¬ er, a clear distinction between lym¬ phocytes and other mononuclear cells, or identification of lymphocyte sub¬ sets, cannot be made by ordinary staining methods. The availability of monoclonal antibodies against lym¬ phocyte surface antigens34 makes it possible to identify and quantify lym¬ phocyte subpopulations by immunohistochemical methods; this has been done in blood, lymph nodes,5 tonsils,6·7 salivary glands,8 gastrointestinal tract,912 kidney,'3 middle ear,14 and skin.15 Lymphocyte subsets have also been characterized in bronchoalveolar lavage fluid,1618 but, to our knowledge, there has been no report on the lymphocyte types and distribution in the normal airway mucosa.We describe the use of monoclonal antibodies to characterize the lym¬ phocyte and monocyte/macrophage populations of the normal human nasal mucosa. This mucous membrane is structurally similar to that of the tracheobronchial tree,19 and the nose provides a readily accessible source of biopsy specimens, sufficient for quan¬ titative characterization of the human airway mucosa. PATIENTS AND METHODS ParticipantsForty healthy adult volunteers older than age 18 years with normal rhinologic examinations and complete blood cell counts were recruited from the Charlottes¬ ville, Va, community (29 women and 11 men; mean age, 31 years; range, 21 to 45 years). Individuals with upper-respiratory illness or fever within one week prior to the study were excluded, as were individu¬ als using intranasal sprays or other drugs, and those with chronic allergic rhinitis, nasal polyps, or asthma. Nasal Biopsy Collection and FixationBiopsy specimens were taken from the anterior part of the inferior turbinate with a 4-mm double-ring biopsy forceps. The biopsy site was anesthetized with a 4% cocaine solution applied on cotton-wool. Hemostasis was obtained by silver nitrate cautery. The biopsy specimens were placed
Background: Hyperglycemia in the adult inpatient population remains a topic of intense study in U.S. hospitals. Most hospitals have established glycemic control programs but are unable to determine their impact. The 2009 Remote Automated Laboratory System (RALS) Report provides trends in glycemic control over 4 years to 576 U.S. hospitals to support their effort to manage inpatient hyperglycemia. Methods: A proprietary software application feeds de-identified patient point-of-care blood glucose (POC-BG) data from the Medical Automation Systems RALS-Plus data management system to a central server. Analyses include the number of tests and the mean and median BG results for intensive care unit (ICU), non-ICU, and each hospital compared to the aggregate of the other hospitals. Results: More than 175 million BG results were extracted from 2006–2009; 25% were from the ICU. Mean range of BG results for all inpatients in 2006, 2007, 2008, and 2009 was 142.2–201.9, 145.6–201.2, 140.6–205.7, and 140.7–202.4 mg/dl, respectively. The range for ICU patients was 128–226.5, 119.5–219.8, 121.6–226.0, and 121.1–217 mg/dl, respectively. The range for non-ICU patients was 143.4–195.5, 148.6–199.8, 145.2–201.9, and 140.7–203.6 mg/dl, respectively. Hyperglycemia rates of >180 mg/dl in 2008 and 2009 were examined, and hypoglycemia rates of <40 mg/dl (severe) and <70 mg/dl (moderate) in both 2008 and 2009 were calculated. Conclusions: From these data, hospitals can determine the current state of glycemic control in their hospital and in comparison to other hospitals. For many, glycemic control has improved. Automated POC-BG data management software can assist in this effort.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.