To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that of cells exposed to protein synthesis inhibitor, erythromycin. Cells were incubated with methionine analog, homopropargyl glycin, and their nascent proteome was selectively labeled with monosulfonated neutral Cy3 and Cy5 azides specially synthesized for this purpose. Following fluorescent labeling, the protein samples were mixed and subjected to standard 2D DIGE separation. The method allowed us to reveal a dramatic reduction of newly synthesized proteins upon erythromycin treatment, while the total proteome was not significantly affected. Additionally, several proteins, whose synthesis was resistant to erythromycin, were identified.
Быканова Н.И., Гордя Д.В., Евдокимов Д.В. Тенденции и закономерности процесса цифровизации банковского сектора Белгородский государственный национальный исследовательский университет ул. Победы,
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The possibility of developing a technique for calculating the wear of the fl ank surface of mills is shown on the basis of a generalized friction model, taking into account the entire complex of the main external factors acting and the physical and mechanical characteristics of the materials of the interacting surfaces of the tool and the workpiece. The calculated dependence of the wear values, the infl uence of some hard-to-consider factors is supposed to be taken into account by the experimentally determined proportionality coeffi cients and the index of the calculated ratio, has been proposed. Keywords: generalized model of friction and wear, the teeth of the mill, the calculated dependence.
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