Aloperine (Alo), as a quinolizidine alkaloid extracted from S. alopecuroide, has the positive activities of anti-inflammatory, anti-allergenic, antitumor and anti-viral. However, the role and mechanism of Alo in breast cancer have not been studied yet. In the present study, Alo markedly inhibited the proliferation and suppressed the colony formation ability of the breast cancer cell lines MCF-7 and MDA-MB-231 in a dose-dependent manner by Cell Counting kit-8 and colony formation assays, respectively. In addition, the results of confocal microscopy analysis and flow cytometry detection revealed that Alo induced the apoptosis of MCF-7 and MDA-MB-231 cells, and western blotting indicated that Alo upregulated the protein levels of Bax, caspase-3 and caspase-9, and downregulated the expression of Bcl-2. Furthermore, the results of wound healing, Transwell migration and invasion assays demonstrated that Alo inhibited the migration and invasion of MCF-7 and MDA-MB-231 cells, and reduced the protein levels of matrix metalloproteinase (MMP)-2 and MMP-9. Alo also downregulated the protein expressions of Ras, phosphorylated (p)-Raf proto-oncogene, serine/threonine kinase 1 and p-extracellular signal-regulated kinase 1/2. Furthermore, ISIS 2503, a Ras inhibitor, inhibited colony formation, induced apoptosis, and suppressed the migration and invasion of MCF-7 and MDA-MB-231 cells. These effects were more marked in the presence of ISIS 2503 and Alo, when compared with those of either agent alone. In conclusion, the present study reported a novel use of Alo in inhibiting the proliferation, migration and invasion, and inducing the apoptosis of human breast cancer cells by blocking the Ras signaling pathway.

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With the implementation of water-conservation projects in the Hetao Irrigation District (HID), great changes have taken place in the ecohydrological process. A cultivated land–wasteland–lake system in the upper Yellow River Basin (YRB) was chosen to study the soil salt and water transport process with the Hydrus-1D model. The model parameters were calibrated and validated by measuring the soil salt and water data. Measured values were in good agreement with the simulated values. The results showed, in the whole growth period, the deep percolation of cultivated land was 34–40% of the total applied water (rainfall and irrigation). The capillary rise in the cultivated land, wasteland, and lake boundary was 24%, 29–35%, and 62–68% of their own evapotranspiration, respectively. The capillary rise in the lake boundary was about 2 times that of the wasteland and 2.6 times that of the cultivated land. The salt storage in the 1 m soil zone of the lake boundary was more than 10% and 18% greater than that of the wasteland and cultivated land, respectively. The salt of the capillary rise in the lake boundary exceeded that of the wasteland by a factor of three. The salt accumulation in the upper soil zone of the cultivated land, wasteland, and lake boundary was 13%, 37%, and 48%. Soil salinization in the upper soil zone of the wasteland and lake boundary was serious, and some measures should be taken to reduce the salt content to prevent soil salinization. The results act as a theoretical basis for the ecohydrological control of the HID.