Non-point source (NPS) pollution is a major cause of the deterioration of surface water quality. Effectively controlling NPS pollution especially that from rural areas, is critical in improving the quality and safety of water resources. However, most of the approach employed for NPS control is low-efficient or impractical owing to ignorance of the willingness of participants, as well as the high cost. To improve the effectives and efficiency, this study newly proposes a resource-based approach for agricultural NPS pollution control via using the biological agents. In this approach, the domestic organic waste and livestock manure are economically utilized to produce organic fertilizers and plant protection agents to replace harmful chemical fertilizers and pesticides, respectively. The approach has been applied in the Danjiangkou catchment area in Nanyang City, and the results show that: (1) the most severe pollutants, total P and total N concentrations have been effectively reduced and their values in 2025 are predicted to be the same level as that before significant deterioration; (2) with unpowered sewage treatment and spontaneous participation of farmers, the treatment cost is significantly reduced; (3) the resource-based treatment of domestic waste and livestock manure supports the development of an efficient and sustainable agricultural economy.
Studies have shown that abundant and various flavonoids accumulate in chili pepper (Capsicum), but there are few reports on the genes that govern chili pepper flavonoid biosynthesis. Here, we report the comprehensive identification of genes encoding type III polyketide synthase (PKS), an important enzyme catalyzing the generation of flavonoid backbones. In total, 13, 14 and 13 type III PKS genes were identified in each genome of C. annuum, C. chinense and C. baccatum, respectively. The phylogeny topology of Capsicum PKSs is similar to those in other plants, as it showed two classes of genes. Within each class, clades can be further identified. Class II genes likely encode chalcone synthase (CHS) as they are placed together with the Arabidopsis CHS gene, which experienced extensive expansions in the genomes of Capsicum. Interestingly, 8 of the 11 Class II genes form three clusters in the genome of C. annuum, which is likely the result of tandem duplication events. Four genes are not expressed in the tissues of C. annuum, three of which are located in the clusters, indicating that a portion of genes was pseudogenized after tandem duplications. Expression of two Class I genes was complementary to each other, and all the genes in Class II were not expressed in roots of C. annuum. Two Class II genes (CA00g90790 and CA05g17060) showed upregulated expression as the chili pepper leaves matured, and two Class II genes (CA05g17060 and CA12g20070) showed downregulated expression with the maturation of fruits, consistent with flavonoid accumulation trends in chili pepper as reported previously. The identified genes, sequences, phylogeny and expression information collected in this article lay the groundwork for future studies on the molecular mechanisms of chili pepper flavonoid metabolism.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.