In a two-phase anaerobic digestion process, enhanced biogas production and organic pollutant removal depend on the stability and performance of the hydrolytic–acidogenic and methanogenic phases. Additionally, the hydrolytic–acidogenic phase is a rate-limiting step, which calls for the further optimization of operating parameters. The objective of this study was to optimize the operating parameters of the hydrolytic–acidogenic reactor (HR) in the two-phase anaerobic digestion treating slaughterhouse wastewater. The experiment was carried using bench-scale sequential bioreactors. The hydrolytic–acidogenic reactor operating parameters were optimized for six different hydraulic retention times (HRTs) (6–1 day) and organic loading rates (OLRs) (894.41 ± 32.56–5366.43 ± 83.80 mg COD/L*day). The degree of hydrolysis and acidification were mainly influenced by lower HRT (higher OLR), and the highest values of hydrolysis and acidification were 63.92% and 53.26% at an HRT of 3 days, respectively. The findings indicated that, at steady state, the concentrations of soluble chemical oxygen demand (SCOD) and total volatile fatty acids (TVFAs) decrease as HRT decreases and OLR increases from HRTs of 3 to 1 day and 894.41–1788.81 mg COD/L*day, respectively, and increase as the HRT decreases from 6 to 4 days. The concentration of NH4+-N ranges from 278.67 to 369.46 mg/L, which is not in the range that disturbs the performance and stability of the hydrolytic acidogenic reactor. It was concluded that an HRT of 3 days and an ORL of 1788.81 mg COD/L*day were selected as optimal operating conditions for the high performance and stability of the two-phase anaerobic digestion of slaughterhouse wastewater in the hydrolytic–acidogenic reactor at a mesophilic temperature. The findings of this study can be applicable for other agro-process industry wastewater types with similar characteristics and biowaste for value addition and sustainable biowaste management and safe discharge.
Background : Wastewater from agro-industries such as slaughterhouse is typical organic wastewater with high value of biochemical oxygen demand, chemical oxygen demand, biological organic nutrients (Nitrogen and phosphate) which are insoluble, slowly biodegradable solids, pathogenic and non-pathogenic bacteria and viruses, parasite eggs. Moreover it contains high protein and putrefies fast leading to environmental pollution problem. This indicates that slaughterhouses are among the most environmental polluting agro-industries. Anaerobic digestion is a sequence of metabolic steps involving consortiums of several microbial populations to form a complex metabolic interaction network resulting in the conversation of organic matter into methane (CH 4 ), carbon dioxide (CO 2 ) and other trace compounds. Separation of the phase permits the optimization of the organic loading rate and HRT based on the requirements of the microbial consortiums of each phase. The purpose of this study was to optimize the working conditions for the hydrolytic - acidogenic stage in two step/phase anaerobic digestion of slaughterhouse wastewater. The setup of the laboratory scale reactor was established at Center for Environmental Science, College of Natural Science with a total volume of 40 liter (36 liter working volume and 4 liter gas space). The working parameters for hydrolytic - acidogenic stage were optimized for six hydraulic retention time 1-6 days and equivalent organic loading rate of 5366.43 – 894.41 mg COD/L day to evaluate the effect of the working parameters on the performance of hydrolytic – acidogenic reactor. Result : The finding revealed that hydraulic retention time of 3 day with organic loading rate of 1,788.81 mg COD/L day was a as an optimal working conditions for the parameters under study for the hydrolytic - acidogenic stage. The degree of hydrolysis and acidification were mainly influenced by lower hydraulic retention time (higher organic loading rate) and highest values recorded were 63.92 % at hydraulic retention time of 3 day and 53.26% at hydraulic retention time of 2 day respectively. Conclusion : The finding of the present study indicated that at steady state the concentration of soluble chemical oxygen demand and total volatile fatty acids increase as hydraulic retention time decreased or organic loading rate increased from 1 day hydraulic retention time to 3 day hydraulic retention time and decreases as hydraulic retention time increase from 4 to 6 day. The lowest concentration of NH 4 + -N and highest degree of acidification was also achieved at hydraulic retention time of 3 day. Therefore, it can be concluded that hydraulic retention time of 3 day/organic loading rate of 1,788.81 mg COD/L .day was selected as an optimal working condition for the high performance and stability during the two stage anaerobic digestion of slaughterhouse wastewater for the hydrolytic-acidogenic stage under mesophilic temperature range selected (37.5℃). Keywords : Slaughterhouse Wastewater, Hydrolytic – Acidogenic, Two Phase Anaerobic Digestion, Optimal Condition, Agro-processing wastewater
The objective of the present study was an optimization of operating parameters and the performance of the methanogenesis reactor in phased anaerobic digestion (AD) of slaughterhouse wastewater at 37.5°C. Accordingly, the feedstock of the methanogenic reactor was effluent from the hydrolytic-acidogenic reactor operating at HRT of 3-days and OLR of 1789 mg/L. The methanogenesis phase was also investigated at different hydraulic retention time (HRT) values ranging from 12 to 3 days at 3-day intervals, and organic loading rates (OLR) of 149, 199, 298, and 596 mg of COD/L. The methanogenesis reactor effluent concentrations of TN, TP, PO4− 3, SO4− 2, and S2− 2 were ranging between 424–464, 83–117, 63–86, 130–197, and 0.98–1.02 mg/L, respectively. The removal efficiencies of TN and TP were vary from 10–17% to 17–21%, respectively. The average biogas production was 125 ± 16, 150 ± 10, 185 ± 4, and 154 ± 17 mL at HRT of 12, 9, 6, and 3 days, respectively. Methane quality (%) and yield (mg/L of COD) were 55–67% and 0.02–0.03, respectively. Furthermore, the average stability indicator parameter values of (total volatile fatty acid (TVFA) = 520 ± 19 mg/L, total alkalinity (TotA) = 1424 ± 10 mg/L, TVFA:TotA. Ratio = 0.36, salinity = 1172 mg/L, pH = 6.92) and performance indicator parameters removal efficiency (RE) for (chemical oxygen demand (COD) = 81%, volatile solid (VS) RE = 95%, biogas production = 185 ± 4 mL, methane yield = 0.03 per mg COD consumed) were achieved at HRT of 6 days and OLR of 298 mg of COD/L. Low removal efficiencies of TP and TN at all HRT/OLR were observed for the methanogenic reactor signifying further treatment system. Graphical Abstract
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.