Leaf leachates of two cultivars of potato i.e. Kufri Chandramukhi (KCM) and Kufri Jyoti (KJ ) contain nine amino acids. Total sugar content of KCM and KJ leachates varied between 0.10 and 0.37 µg per sq. cm and 0.02 to 0.23 µg per sq. cm. respectively. The sugars exuded more by KCM. Total phenol content of KCM and KJ leachates varied between 1.00 to 2.0 µg per sq. cm and 1.05 to 3.16 µg per sq. cm respectively. The percentage germination of P. infestans sporangia in KCM and KJ cultivars was more in mature leaf leachates as compared to young leaves, moreover it was less in KJ cultivar as compared to KCM. The germ tube length of P. infestans zoospores in leaf leachates was inhibited in both the cultivars except in mature leaf leachate of 94 days old Ku fri Chandramukhi.
The active components of stem bark of Tinospora cordifolia were extracted using cold water and organic solvents (methanol, diethyl ether and acetone) and were tested against Staphylococcus aureus using the agar disc diffusion method. All the four extracts inhibited the growth of S. aureus, with methanol extract exerting the highest activity whereas water extract was least active. The results were compared with the reference antibiotic ciprofloxacin
The in vitro antibacterial activity of Rauwolfia serpentina plant extract has been investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae using the disc diffusion method. The alcoholic extract was found effective against Staphylococcus aureus only. The antibacterial activity is attributed to the presence of alkaloids, which was confirmed by gas liquid chromatography and positive alkaloid test. The minimum inhibitory concentration (MIC) was determined by paper disc diffusion method. The results were compared with reference antibiotic tetracycline (one unit solution).
Methanol, diethyl ether and acetone extracts of leaf of itex negundo were tested for their antibacterial activity against two human pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa and one fungus Aspergillus niger using the disc diffusion method. It was found that all the extracts produced inhibitory effect but the methanol extract of leaves exhibited a superior level of antimicrobial activity. The minimum inhibitory concentration(M IC) and minimum fungicidal concentration(MFC)was determined by broth dilution method. The results were compared with the reference antibiotics.
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