Teeth are the most resistant and hardest structures of human body which are usually better preserved than other parts of skeleton and maintain their forensic odontological significance for a comparatively longer period of time. They retain their unique features even in the worst environmental conditions from taphonomic degradations to biological or chemical destructions. They can survive all sorts of natural or man-made disasters and the taphonomic destructions. Besides the unique odontological, molecular and chemical characteristics of teeth; the dental pathologies and structural anomalies also play crucial role in forensic identification of unknown human skeletal remains. The idiosyncratic features like tooth staining/coloration patterns, developmental defects, tooth wear and attritions, dental restorations/implants, cultural tooth modifications, tobacco or nut-chewing signs, occupational stigmas etc., act as valuable adjuncts to forensic examination of teeth found in forensic or bioarchaeological contexts. Dental pathologies and anomalies may reflect the oral hygiene, dietary patterns (like consumption of sweets and sugar, fats, proteins) socio-economic or socio-cultural, and the occupational status of an individual. Present review article presents a brief overview of different dental defects and their putative role in forensic anthropological identification of unknown human remains.
In 2014, 157 years after the Sepoy Mutiny of 1857, several unidentified human skeletons were discovered in an abandoned well at Ajnala, Punjab. The most prevailing hypothesis suggested them as Indian soldiers who mutinied during the Indian uprising of 1857. However, there is an intense debate on their geographic affinity. Therefore, to pinpoint their area of origin, we have successfully isolated DNA from cementum-rich material of 50 good-quality random teeth samples and analyzed mtDNA haplogroups. In addition to that, we analyzed 85 individuals for oxygen isotopes (δ18O values). The mtDNA haplogroup distribution and clustering pattern rejected the local ancestry and indicated their genetic link with the populations living east of Punjab. In addition, the oxygen isotope analysis (δ18O values) from archaeological skeletal remains corroborated the molecular data and suggested the closest possible geographical affinity of these skeletal remains toward the eastern part of India, largely covering the Gangetic plain region. The data generated from this study are expected to expand our understanding of the ancestry and population affinity of martyr soldiers.
Stable isotope analysis of biogenic tissues like tooth and bone has become a widely recognized and increasingly important method for provenance of human remains, particularly in bio-archaeological and forensic investigations. Establishing the dietary status and identity of unknown human skeletal remains retrieved from forensic anthropological contexts is a challenging task. Thousands of unknown human osseous remains along with the personalized contextual items, reportedly belonging to 282 Indian soldiers killed in 1857, were excavated non-scientifically from an abandoned well at Ajnala (Amritsar, India). In present study, the isotopic concentrations of carbon (C) and nitrogen (N) were estimated from the dentine collagen extracted from 21 first molars to provide information about the geographic affinity and dietary status of the individuals killed in Ajnala. As diagenesis affects bone more severely than enamel or dentine due to increased porosity of the former, so teeth were preferred to bones for stable isotope analysis in present study. The literature supported C: N range of 2.8-3.6 was considered as cut-off for the well-preserved collagen and the samples with values outside this range were considered to be altered or contaminated with non-collagenous materials. The interpretation of the obtained isotope values from Ajnala teeth samples indicated the consumption of C3/C4 mixed diet (though some samples showing marine diet) by the victims which supported the previous observations about the dietary status of Ajnala victims estimated from prevalence of various dental pathologies. Though C and N isotopes are generally not the best indicators of geographic origin, they can be used to for the purpose only if they show different dietary inputs of C3 and C4 plants. Present study results provided scientific confirmation to the written historical accounts that Ajnala skeletal remains belonged to the individuals belonging to the Indian states of Awadh (north-eastern Uttar Pradesh), Bihar and Bengal and some norteastern states.
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