The study was carried out to investigate the responses of drought stress on wheat tissues. Five wheat cultivars Alta 84 (AL), Atila (AT), Cettia (CE), Norman Bourgluk 2008 (NB) and Seri M82 (SE) were cultivated. Four weeks after planting, healthy plants were subjected to four treatments viz-well-watered (D1), watered daily, drought stress (D2), one weekly watering, drought stress (D3), two weekly watering and drought stress (D4), and three weekly watering. The experiment was a factorial experiment conducted in a completely randomized design (CRD) with three replicates; Transverse section (TS) of the leaves collected were sectioned using microtome, stained and observed under compound microscope. Mesophyll cells, vascular bundles, bundle sheaths and xylem tissues were measured using ocular micrometer. Epidermal peels were prepared to determine the number of stomata and trichomes. When drought stress was imposed (D2, D3 and D4), wheat cultivar Cettia developed larger mesophyll cells, xylem tissues, vascular bundles and bundle sheaths than other cultivars. Thus, larger vascular bundles would facilitate faster movement of water and mineral salts up to the shoot from the root. Cultivar Atila developed higher number of trichomes during drought stress D2 than other cultivars which help to reduce the rate of transpiration of water out of the epidermal leaves. Wheat cultivars Cettia and Atila could be drought tolerant cultivars. Generally, wheat cultivars developed stable stomata number and stomata closure mechanism to cope with drought stress. There was an increase in number of trichomes at D2, D3 and D4 which revealed the ecologically adaptation of wheat plants to drought stress.
The relationship between eleven Tephrosia species occurring in Nigeria was examined using a number of taxonomic tools. Fresh and herbarium specimens were used for this purpose and methods followed conventional taxonomic practice. Although the species occur in savanna ecosystems, herbarium collections revealed an abundant distribution in the southern part of Nigeria. Morphometric studies revealed that four quantitative characters viz: leaflet length, lamina length, fruit length and pedicel length can be used to delimit members of this genus. Based on the cluster analysis using average linkage within group, the closest species are T. leptostachya and T. purpurea with the shortest distance measure (0.713). T. linearis has the smallest leaflet, in length and width. Further morphological studies also showed that T. vogelii has the largest pod while T. barbigera has the highest number of seeds per pod. Generally, the species have very short petiolule ranging between 0.2cm and 0.3cm in length, on the average. Foliar micro-morphological studies also showed that the species generally possess polygonal cells with straight to curved anticlinal walls and anisocytic stomata types while pollen studies revealed tricolporate pollen grains to be predominant within the taxa. Although, the present study has added to the existing information regarding Tephrosia species, it also suggests further research to ascertain its taxonomic position within the Papilionaceae.
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