The effect of actinomycin D on the sensitivity of endotoxin-responsive (C3HeB/FeJ) and endotoxin-unresponsive (C3H/HeJ) mice to challenge with purified lipopolysaccharide (LPS) of Escherichia coli strain O55:B5 was examined using an experimental protocol of adoptive transfer of lymphoid cells into lethally irradiated recipients. Earlier results--that in the absence of actinomycin D, the ability of LPS to cause a lethal response in the immunologically chimeric mice reflected the phenotypic response of the donor lymphoid cells--were confirmed. Simultaneous administration of actinomycin D to endotoxin-responsive C3HeB/FeJ mice increased by several orders of magnitude the sensitivity of these mice to the lethal effects of LPS. Determinations of 50% lethal doses in the presence of actinomycin D indicated that immunologic chimeras were sensitive to lethal effects of LPS if either the donor or the recipient phenotype was LPS-responsive. Thus, the mechanism(s) of host response to LPS in the presence of actinomycin D may not be identical to those elicited in untreated mice.

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The Eastern Coal Province is divided into 24 separate reporting areas. The divisions are based upon hydrologic factors, location, size, and mining activity. Hydrologic units (drainage basins) or parts of units are combined to form each area. Area 5, in the northern part of the Eastern Coal Province, is comprised of the 7,384 square-mile Monongahela River basin. Recent streamflow data have been collected at 50 continuous-record gaging stations, 4 crest-stage partial-record stations, and 13 low-flow partialrecord stations in addition to 126 miscellaneous sites. Low-flow, mean-flow, peak-flow, and flow-duration data are presented for gaging stations in Area 5. Techniques and sources of information are presented to enable estimates of these flow characteristics at ungaged sites. Water levels in observation wells in Area 5 fluctuate throughout the year. Levels are generally lowest during late summer and early fall and highest during early spring. The U.S. Geological Survey identifies and improves access to existing water data through: the National Water Data Exchange, the National Water Data Storage and Retrieval System, and the Office of Water Data Coordination.

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