Microbial communities in the subterranean environment are unique in that they are completely isolated from biological communities that rely on photosynthesis, earth's atmosphere or oceans. Interestingly, bacteria found in petroleum deposits are often in a dormant form because petroleum deposits are very limited in nitrogenous and phosphorous-containing nutrients. These are unique populations as compared to other subsurface microbial communities and might consist of novel species, new metabolic capabilities, and undiscovered adaptive microbial mechanisms. The goal of the current study was to isolate and identify cultivable bacteria (mainly oligotrophs) and develop a method to evaluate bacterial community structure in petroleum deposits using 16S rDNA based technique.
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