ContextA landscape is defined as a "system of ecosystems" and this is a model in which karst areas can easily be integrated. In karst areas, much of the connectivity between the units of the landscape is underground, with aquifers and caves forming a continuous layered tissue. However, underground environments are among the least studied landscapes on Earth because of limited accessibility and the difficulty of performing surveys. ObjectivesThe aim of this paper is to provide a conceptual framework for applying principles of landscape ecology to research on karst environments. MethodsBy adapting the standard patch-corridor-matrix model to a 3d model, the main issues that need to be addressed were identified. These include identifying the main morphological (surface and underground) karst features; determining the landscape structure through its features, composition, and configuration; and developing adequate indices. ResultsThe landscape spatial structure of different karst areas influences fundamental ecological functions and biodiversity patterns. Determining how structure, biodiversity, and functions relate reveals important insights 2 into the functioning of karst systems. Emphasizing the provisioning of ecosystem services is essential in supporting the concept that karst regions are vital for human well-being because they host valuable resources and fundamental ecosystem processes. The paper discusses how this framework helps address anthropogenic impacts and conservation issues on karst. ConclusionsThe potential of applying a landscape approach to karst systems lies in developing models that provide ecological information relevant to understanding karst systems and understanding their implications for natural resources management.