The fungal skin disease, chytridiomycosis (caused by Batrachochytrium dendrobatidis and B. salamandrivorans), has caused amphibian declines and extinctions globally since its emergence. Characterizing the host immune response to chytridiomycosis has been a focus of study with the aim of disease mitigation. However, many aspects of the innate and adaptive arms of this response are still poorly understood, likely due to the wide range of species' responses to infection. In this paper we provide an overview of expected immunological responses (with inference based on amphibian and mammalian immunology), together with a synthesis of current knowledge about these responses for the amphibian-chytridiomycosis system. We structure our review around four key immune stages: (1) the naïve immunocompetent state, (2) immune defenses that are always present (constitutive defenses), (3) mechanisms for recognition of a pathogen threat and innate immune defenses, and (4) adaptive immune responses. We also evaluate the current hot topics of immunosuppression and immunopathology in chytridiomycosis, and discuss their respective roles in pathogenesis. Our synthesis reveals that susceptibility to chytridiomycosis is likely to be multifactorial. Susceptible amphibians appear to have ineffective constitutive and innate defenses, and a late-stage response characterized by immunopathology and Bd-induced suppression of lymphocyte responses. Overall, we identify substantial gaps in current knowledge, particularly concerning the entire innate immune response (mechanisms of initial pathogen detection and possible immunoevasion by Bd, degree of activation and efficacy of the innate immune response, the unexpected absence of innate leukocyte infiltration, and the cause and role of late-stage immunopathology in pathogenesis). There are also gaps concerning most of the adaptive immune system (the relative importance of B and T cell responses for pathogen clearance, the capacity and extent of immunological memory, and specific mechanisms of pathogen-induced immunosuppression). Improving our capacity for amphibian immunological research will require selection of an appropriate Bd-susceptible model species, the development of taxon-specific affinity reagents and cell lines for functional assays, and the application of a suite of conventional and emerging immunological methods. Despite current knowledge gaps, immunological research remains a promising avenue for amphibian conservation management.

The Global Precipitation Measurement (GPM) mission is an international satellite mission that uses measurements from an advanced radar/radiometer system on a core observatory as reference standards to unify and advance precipitation estimates made by a constellation of research and operational microwave sensors. The GPM core observatory was launched on February 27, 2014 at 18:37 UT in a 65 • inclination nonsun-synchronous orbit. GPM focuses on precipitation as a key component of the Earth's water and energy cycle, and has the capability to provide near-real-time observations for tracking severe weather events, monitoring freshwater resources, and other societal applications. The GPM microwave imager (GMI) on the core observatory provides the direct link to the constellation radiometer sensors, which fly mainly in polar orbits. The GMI sensitivity, accuracy, and stability play a crucial role in unifying the measurements from the GPM constellation of satellites. The instrument has exhibited highly stable operations through the duration of the calibration/validation period. This paper provides an overview of the GMI instrument and a report of early on-orbit commissioning activities. It discusses the on-orbit radiometric sensitivity, absolute calibration accuracy, and stability for each radiometric channel.Index Terms-Calibration accuracy, passive microwave remote sensing, radiometric sensitivity.

The impacts of pathogen emergence in naïve hosts can be catastrophic, and pathogen spread now ranks as a major threat to biodiversity. However, pathogen impacts can persist for decades after epidemics and produce variable host outcomes. Chytridiomycosis in amphibians (caused by the fungal pathogen Batrachochytrium dendrobatidis, Bd) is an exemplar, with impacts ranging from rapid population crashes and extinctions, to population declines and subsequent recoveries. Here, we investigate long-term impacts associated with chytridiomycosis in Australia. We conducted a continent-wide assessment of the disease, reviewing data collected since the arrival of Bd in about 1978, to assess and characterize mechanisms driving past, present and future impacts. We found chytridiomycosis to be implicated in the extinction or decline of 43 of Australia's 238 amphibian species. Population trajectories of declined species are highly variable; six species are experiencing ongoing declines, eight species are apparently stable and 11 species are recovering. Our results highlight that while some species are expanding, Bd continues to threaten species long after its emergence. Australian case-studies and synthesis of the global chytridiomycosis literature suggests that amphibian reservoir hosts are associated with continued declines in endemically infected populations, while population stability is promoted by environmental conditions that restrict Bd impact, and maintenance of high recruitment capacity that can offset mortality. Host genetic adaptation or decreased pathogen virulence may facilitate species recovery, but neither has been empirically demonstrated. Understanding processes that influence Bd-host dynamics and population persistence is crucial for assessing species extinction risk and identifying strategies to conserve disease-threatened species.

Summary1. The cane toad (Rhinella marina) invasion of Australia has now reached areas much colder than most of its native range in tropical America. Understanding the toad's ability to function in such conditions can clarify its potential for further spread. 2. In northeastern New South Wales (NSW), cane toads have been recorded up to 1100 m above sea level (asl). Our monitoring over summer 2012-2013 confirmed that ground temperatures were lower at three high-elevation (750-1010 m asl) sites than at two nearby lower (100-210 m asl) sites (by day, 18°C vs. 25°C; at night, 17-18°C vs. 20-25°C, respectively). 3. Critical thermal minima (CTmins) of field-collected toads (loss of the righting reflex) were lower for high-elevation than low-elevation toads (5Á5°C vs. 7Á5°C), but laboratory acclimation abolished this difference. A toad's CTmin was not affected by site of collection, nor by 1 month's exposure to warm (24°C) or cool (12°C) conditions; instead, a toad's CTmin was determined by its thermal exposure over the previous 12 h. Locomotor ability was affected by test temperature, by elevation and by acclimation. Toads from high elevations exhibited equal endurance at cold and warm test temperatures after month-long acclimation to cold conditions, whereas toads from low elevations performed better at high temperatures regardless of previous temperature treatments. 4. Cane toads at the southern edge of their expanding Australian range can function under cool conditions by adjusting their thermal tolerance within a few hours of encountering low temperatures. 5. The toads' ability for rapid thermal acclimation suggests that current models underestimate the potential range of abiotic conditions accessible to this invading species.

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