BackgroundAddiction and major depression are mental health problems associated with stressful events in life with high relapse and reoccurrence even after treatment. Many laboratories were not able to detect the presence of cannabinoid CB2 receptors (CB2-Rs) in healthy brains, but there has been demonstration of CB2-R expression in rat microglial cells and other brain associated cells during inflammation. Therefore, neuronal expression of CB2-Rs had been ambiguous and controversial and its role in depression and substance abuse is unknown.Methodology/Principal FindingsIn this study we tested the hypothesis that genetic variants of CB2 gene might be associated with depression in a human population and that alteration in CB2 gene expression may be involved in the effects of abused substances including opiates, cocaine and ethanol in rodents. Here we demonstrate that a high incidence of (Q63R) but not (H316Y) polymorphism in the CB2 gene was found in Japanese depressed subjects. CB2-Rs and their gene transcripts are expressed in the brains of naïve mice and are modulated following exposure to stressors and administration of abused drugs. Mice that developed alcohol preference had reduced CB2 gene expression and chronic treatment with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but not in control mice. The direct intracerebroventricular microinjection of CB2 anti-sense oligonucleotide into the mouse brain reduced mouse aversions in the plus-maze test, indicating the functional presence of CB2-Rs in the brain that modifies behavior. We report for the using electron microscopy the sub cellular localization of CB2-Rs that are mainly on post-synaptic elements in rodent brain.Conclusions/SignificanceOur data demonstrate the functional expression of CB2-Rs in brain that may provide novel targets for the effects of cannabinoids in depression and substance abuse disorders beyond neuro-immunocannabinoid activity.

Major depression and addiction are mental health problems associated with stressful events in life with high relapse and reoccurrence even after treatment. Many laboratories were not able to detect the presence of CB2 cannabinoid receptors (CB2-Rs) in healthy brains, but there has been demonstration of CB2-R expression in rat microglial cells and other brain associated cells during inflammation. Therefore, neuronal expression of CB2-Rs had been ambiguous and controversial and its role in depression and substance abuse is unknown. In this study we tested the hypothesis that genetic variants of CB2 gene might be associated with depression in a human population and that alteration in CB2 gene expression may be involved in the effects of abused substances including opiates, cocaine and ethanol in rodents. Here we demonstrate that a high incidence of (Q63R) but not (H316Y) polymorphism in the CB2 gene was found in Japanese depressed subjects. CB2-Rs and their gene transcripts are expressed in the brains of naïve mice and are modulated following exposure to stressors and administration of abused drugs. Mice that developed alcohol preference had reduced CB2 gene expression and chronic treatment with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but not in control mice. The direct intracerebroventricular microinjection of CB2 anti-sense oligonucleotide into the mouse brain reduced mouse aversions in the plus-maze test, indicating the functional presence of CB2-Rs in the brain that modifies behavior. Using electron microscopy we report the sub cellular localization of CB2-Rs that are mainly on post-synaptic elements in rodent brain. Our data demonstrate the functional expression of CB2-Rs in brain that may provide novel targets for the effects of cannabinoids in depression and substance abuse disorders beyond neuro-immunocannabinoid activity.

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Results from recent trials have evaluated the potential clinical advantages of metformin in patients with cardiac disease and HF. These studies have illustrated a favorable morbidity and mortality profile for the use of metformin in these patient populations. While large prospective trials are still needed to display conclusive evidence, the recent clinical trials suggest a benefit in areas where metformin use has previously been discouraged.

Beans (Phaseolus vulgaris L.) are important legumes in the semi-arid areas of Eastern Kenya. But their production is constrained by water stress within the season and between seasons. A field trial was conducted in the short rains in the year 2011 (October to December) and long rains of 2012 (March to June) at KARI-Katumani (10 35'S and 37014'E, and 1560 metres above mean sea level) to determine the effect of on soil moisture and soil nutrients on grain yield of three market -preferred bean genotypes; namely: Katumani bean 1, NUA 1 and NUA 4. The experiment was conducted using a randomized block design with three replications with treatments in split plot arrangement. Results showed that bean grain yields of Kat B1 and NUA1 grown on tied ridges with manure at 5 tons ha-1 produced significantly (P<0.05) higher yields than furrow or flat tillage beans with and without manure in the short rains of 2011. In the long rains of 2012, Kat B1 and NAU1 beans grown on tied ridges with manure out yielded all the other treatments. In the same season, grain yield of NUA 4 grown on tied ridges with manure, was higher than beans grown on open furrows, flat tillage with and without manure. In both seasons, Kat B1 produced significantly higher grain yields than all the other genotypes except NUA 4 during the short rains of 2011.

<span lang="EN-US">Globally, cervical cancer continues to be a major cause of mortality and morbidity among women with developing countries accounting for more than 86% of the deaths from the disease. In Kenya, cervical cancer ranks as the second most frequent cancer among women: regrettably, with 80% of the cases presented during the late stages of the disease when the scope of successful treatment is limited. An opportunity to prevent occurrence of cervical cancer exists through cervical cancer screening for early detection and treatment of precancerous lesions before these develop to cancer. However, only a negligible proportion of women benefit from cervical cancer screening services in developing countries, including Kenya. The purpose of this descriptive survey study was to determine the factors influencing utilization of cervical cancer screening services; and focused on the women seeking maternal and family planning services at Central Provincial General Hospital, Kenya. The study established that, as in most developing countries, utilization of cervical cancer screening services was low, regardless of the women’s education level, autonomy in decision making and even good family support. The low level of awareness on the need for screening services and the long waiting time at the clinic for those seeking the services were identified as the two key issues that negatively influenced the utilization of the screening services, It is therefore recommended that the Ministry of Health and other agencies, including individuals of good will, collaborate in designing and implementing awareness campaigns through the media and other appropriate avenues, including one-to-one encounters, targeting both men and women in the communities. It is also recommended that the male partners support their women in accessing this vital life-saving service.</span>

In order to improve efficiency in health service delivery in Kenya, the Ministry of Health has developed structures through inter-sectoral collaboration at various levels. Despite efforts by the government and key stakeholders to improve health sector, utilization of health services still remains a major challenge. The purpose of the study was to investigate factors that influence utilization of health services in Homa Bay County, Kenya. The study employed survey design and focused on health beneficiaries, District Health Management Team and other key health stakeholders, and used both quantitative and qualitative data. Quantitative data was collected through household interviews of 384 respondents and qualitative data was generated through Key Informant Interviews of 16 respondents. The study reveals that health financing, service delivery, quality, accessibility and equity influence utilization of health services in Homa Bay County. It is for these reasons that the study recommends that the government should allocate adequate budget towards health services, avail adequate trained health workers, and improve infrastructure in health facilities as well as drugs and other supplies. There is also need for further research on cultural factors influencing utilization of health services.

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