We report the design and operation of a versatile tunnel diode oscillator for high sensitivity ( ≈ 50 ppb) measurements of the magnetic susceptibility of samples over a wide temperature range (1.7-100 K) that can be used with a simple liquid helium storage dewar. The design allows for the application of an electric field across the sample to search for magnetoelectric effects.
Ponazuril (toltrazuril sulfone) is a triazine antiprotozoal agent that targets apicomplexan organisms. Ponazuril may have clinical application in the treatment of clinical coccidiosis due to Eimeria species in goats, along with other protozoal infections. To evaluate the absorption, distribution and elimination characteristics of ponazuril in goats, a sensitive, validated high-pressure liquid chromatography and mass spectroscopy method for ponazuril in caprine plasma was developed. After a single oral dose of ponazuril at 10 mg/kg, plasma samples from seven weanling goats were collected and assayed. Plasma concentrations of ponazuril in the goats peaked at 36 ± 13 h post drug administration at a concentration of 9 ± 2 μg/mL. Concentrations declined to an average of 4.2 ± 0.8 μg/mL after 168 h with an average elimination half-life of 129 ± 72 h post drug administration. This study shows that ponazuril is relatively well absorbed after a single oral dose in goats. Efficacy trials are underway to determine clinical efficacy of ponazuril in the treatment of clinical coccidiosis in goats at 10 mg/kg dosage.
Background Tritrichomonas foetus is a sexually transmitted protozoon that causes reproductive failure, among cattle, so disruptive that many western US states have initiated control programs. Current control programs are based on the testing and exclusion of individual bulls. Unfortunately, these programs are utilizing screening tests that are lacking in sensitivity. Blanket treatment of all the exposed bulls and adequate sexual rest for the exposed cows could provide a more viable disease control option. The objectives of this study were twofold. The first objective was to demonstrate effectiveness for metronidazole treatment of a bull under ideal conditions and with an optimized treatment regime. This type of study with a single subject is often referred to as an n-of-1 or single subject clinical trial. The second objective of the current study was to review the scientific basis for the banning of metronidazole for use in Food Animals by the Animal Medicinal Drug Use Clarification Act of 1994 (AMDUCA). Results Results from an antimicrobial assay indicated that metronidazole at a concentration of 0.5 μg/mL successfully eliminated in vitro protozoal growth of bovine Tritrichomonas foetus. The estimated effective intravenous dose was two treatments with 60 mg/kg metronidazole, 24 h apart. A bull that had tested positive for Tritrichomonas foetus culture at weekly intervals for 5 weeks prior to treatment was negative for Tritrichomonas foetus culture at weekly intervals for five consecutive weeks following this treatment regimen. An objective evaluation of the published evidence on the potential public health significance of using metronidazole to treat Tritrichomonas foetus in bulls provides encouragement for veterinarians and regulators to consider approaches that might lead to permitting the legal use of metronidazole in bulls. Conclusion The study demonstrated successful inhibition of Tritrichomonas foetus both in vitro and in vivo with metronidazole. The current status of metronidazole is that the Animal Medicinal Drug Use Clarification Act of 1994 prohibits its extra-label use in food-producing animals. Veterinarians and regulators should consider approaches that might lead to permitting the legal use of metronidazole in bulls.
The extraction of sucrose in a sugarcane diffuser depends on the percolation rate of juice through the cane bed. High percolation rates promote mass transfer and increase the wetness of the cane bed (i.e. liquid hold-up within the bed) thereby improving sucrose extraction. However, increasing the rate of juice applied to the surface of the cane bed above the maximum percolation rate results in flooding, causing uncontrolled mixing of juice, destruction of the dry substance content profile and reduced extraction. Flooding in the diffuser can be avoided by installing feedback control of adjustable sprays that alter the application area of juice onto the bed surface and automatically keeping the percolation rate optimised. Electrical conductivity of the cane bed, measured between the bed surface and the bottom screen of the diffuser, has been investigated as a possible online indicator of juice hold-up within the cane bed to provide the necessary measurement for implementing feedback control that can optimise percolation rates. Full scale tests were conducted on the Tongaat Hulett cane diffuser at the Maidstone factory. Reproducibility tests were done to confirm that there is a relationship between conductance and liquid hold-up.
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