The significant worldwide increase in observed river runoff has been tentatively attributed to the stomatal ''antitranspirant'' response of plants to rising atmospheric CO 2 [Gedney N, Cox PM, Betts RA, Boucher O, Huntingford C, Stott PA (2006) Nature 439: 835-838]. However, CO 2 also is a plant fertilizer. When allowing for the increase in foliage area that results from increasing atmospheric CO 2 levels in a global vegetation model, we find a decrease in global runoff from 1901 to 1999. This finding highlights the importance of vegetation structure feedback on the water balance of the land surface. Therefore, the elevated atmospheric CO 2 concentration does not explain the estimated increase in global runoff over the last century. In contrast, we find that changes in mean climate, as well as its variability, do contribute to the global runoff increase. Using historic land-use data, we show that landuse change plays an additional important role in controlling regional runoff values, particularly in the tropics. Land-use change has been strongest in tropical regions, and its contribution is substantially larger than that of climate change. On average, land-use change has increased global runoff by 0.08 mm/year 2 and accounts for Ϸ50% of the reconstructed global runoff trend over the last century. Therefore, we emphasize the importance of land-cover change in forecasting future freshwater availability and climate.atmospheric CO2 ͉ water cycle ͉ climate change ͉ land cover change C limate change and human intervention are expected to strongly alter the global hydrological cycle in the coming decades (1-5). Previous reconstruction of global runoff data suggests that global river runoff increased significantly during the 20th century (2). However, it is difficult to estimate whether this trend in runoff is caused by natural or anthropogenic factors, because the characteristics and dynamic properties of the hydrological cycle depend on many interrelated links among climate, atmosphere, soil, and vegetation dynamics. Long-term changes in runoff depend on the balance of precipitation and evapotranspiration. The latter term is not only driven by climatic factors, such as temperature, wind speed, surface humidity, and solar radiation, but also is modulated by physiological (e.g., stomatal) and structural [e.g., leaf area index (LAI)] components of vegetation. It is well known that stomata respond to elevated atmospheric CO 2 concentrations by partial closure (6). Accordingly, a recent modeling analysis suggested that the rising atmospheric CO 2 concentration is the main driver of the observed increase in continental runoff (1). Nevertheless, the results of the Gedney et al.(1) study should be viewed with caution because only the direct effect of atmospheric CO 2 concentrations on stomatal conductance was considered. Structural changes in vegetation in response to increased productivity under higher atmospheric CO 2 levels, particularly changes in LAI (7), were not taken into account in their study.Land use is another key...