Owl monkeys were inoculated intracerebrally, subcutaneously, and intravenously with JC, BK, or SV40 virus. Two of four adult owl monkeys inoculated with JC virus, a human polyomavirus, developed brain tumors at 16 and 25 months after inoculation, respectively. A grade 3 to grade 4 astrocytoma (resembling a human glioblastoma multiforme) was found in the left cerebral hemisphere and brainstem of one monkey. The second monkey developed a malignant tumor in the left cerebral hemisphere containing both glial and neuronal cell types. Impression smears prepared from unfixed tissue of this tumor showed cells that contained polyomavirus T antigen. Virion antigens were not detected. Tumor cells cultured in vitro also contained T antigen but were negative for virion antigen. Infectious virus was not isolated from extracts of this tumor.

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We studied the frequency of oligoclonal immunoglobulin G bands in the cerebrospinal fluid (CSF) of patients with various neurological diseases. We used a micromethod employing sodium dodecyl sulfate polyacrylamide gel electrophoresis that required only 50 microliters of unconcentrated CSF. Oligoclonal bands were detected in the CSF of 95% of the patients with multiple sclerosis, 90% with subacute sclerosing panencephalitis, and 100% with herpes simplex encephalitis, but less frequently in other central nervous system infections. No oligoclonal bands were detected in the CSF of patients with Parkinson, Huntington, Creutzfeldt-Jakob, or herniated disc diseases. Bands were detected in some patients with Alzheimer disease, cerebrovascular accident, idiopathic vertigo, idiopathic seizures, amyotrophic lateral sclerosis, polyneuropathy, and central nervous system glioma. Patients with other conditions infrequently had positive bands. The determination of oligoclonal bands is a useful aid in the diagnosis of multiple sclerosis, subacute sclerosing panencephalitis, and herpes simplex encephalitis. The presence of oligoclonal bands indicates an immunological response but is not diagnostic for a particular condition.

In a prospective study of 9,503 parturients during the past 47 months, 12.3% were colonized with GBS. 25 infants born to these mothers developed GBS disease (attack r a t e 2.611000 l i v e b i r t h s ) .195 mothers delivered a t 4 3 2 weeks gestation. 664 mothers experienced premature membrane rupture (PROM). Colonization with GBS among these groups of p a r t u r i e n t s = 32% and 20% respectively.The incidence of preterm labor a t < 32 weeks gestation = 5.7% among women colonized with GBS i n comparison to 1.7% among women known not t o be colonized (p C .005). Similarly PROM occurred i n 12.3% of colonized p a t i e n t s vs 6.8% of those not colonized (p <.005).In addition t o these observations we have noted 28% GBS colonization among products of spontaneous abortion s e r i a l l y cultured during the past 12 months.These associations between events leading t o premature termination of pregnancy and colonization with GBS. when compared t o the observed a t t a c k r a t e f o r GBS disease have led us to speculate that the magnitude of the threattheGBS poses to the newborn l i e s more i n its r o l e i n the egress of prematurity than i n its potent i a l f o r infection.ASPIRIN AND ACETAMINOPHEN LEVELS I N CHILDREN WITH REYES SYNDROME. G.C. Rod e r s L.B. Weiner J.A.490 M d i l l a n (spon. by W. H. i e r g~t r o m ) . s U N~, '~p s t a t e Medical Center, Dept. of Pediatrics, Syracuse, New York.The records of 61 pts. with Reyes Syndrome (RS) seen a t the Upstate Medical Center during the past 8 years were reviewed. Measurements of serum s a l i c y l a t e and acetaminophen (APAP) concent r a t i o n s were made a t the time of admission i n 37 and 21 pts. respectively. Measurable levels of s a l i c y l a t e were found i n 28 pts. (76%) with a mean value of 5.5 mgldl (range 0.2 mgldl t o 36 mgldl). S a l i c y l a t e l e v e l s a r e summarized i n the following t a b l e according t o presenting stage.Total I U with measurable l e v e l Mean l e v e l (mgldl) Stage I 22 6 4.3 s t a g e 11 31 17 7.3 Stage 1 1 1 -I V 8 5 1.0 The mean l e v e l s i n t h i s t a b l e a r e not s i g n i f i c a n t l y different. S a l i c y l a t e and APAP levels did not c o r r e l a t e with the degree of l i v e r enzyme elevations, blood ammonia l e v e l s o r phenobarbital half-life. Nine of 22 pts. who expired o r had serious sequelae had admission s a l i c y l a t e levels measured. The mean l e v e l was 8.8 mgldl. In 19 of 39 survivors without sequelae i n whom the s a l icylate l e v e l was measured the mean l e v e l was 4.0 mgldl (pc.1). I n the 21 pts. i n whom admission APAP values were determined, measurable levels were noted i n 7 pts. (mean 11.4 vglml, range 2.2-40 uglml). The high prevalence of measurable s a l i c y l a t e l e v e l s suggests a possible relationship between s a l i c y l a t e ingestion and US. These data a l s o indicate a correlation between s a l i c y l a t e l e v e l and outcome. Between November 1979 and March 1980, t h e stools of 183 nursery patients were studie...