Notes(325 ized, and sterilized by free flowing steam for 10 min. Cells for thejinoculum were grown in Henderson and Snell1 23456 basal medium and harvested after 6-8 hr at 35°. E. coli.-The methods used were similar to those described6 with the exceptions that total volumes of 10 ml/50-ml erlenmeyer flask and incubation on a reciprocating shaker at 28°were employed.Testing Procedures.-Initially, all compounds were tested at final concentrations of 100, 200, 400, and 800 jug/ml, both in the absence and presence of exogenous L-cysteine hydrochloride (1.5 µ / , a level required for about half-maximal growth) with L.mesenteroides. With E. coli similar levels of compound were evaluated but only in the absence of cysteine.
ChemInform Abstract A method for the large scale synthesis of azetidine (V) is described and compared with other syntheses of (V). The key step is the base-mediated ring closure reaction of benzhydrylamine (I) with 1-bromo-3-chloropropane (II), yielding Nbenzhydrylazetidine (III) which is deprotected by hydrogenation. The hydrochloride (IV) is converted to the free base (V) by flash distillation over aqueous potassium hydroxide.
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