The (Na+,k+)ATPase from the rectal gland of Carcharhinus obscurus has been solubilized in Lubrol WX as an active complex containing 379,900 g of protein and 61 mol of phospholipid. This detergent-lipid-protein complex contains two catalytic subunits of molecular weight 106,400 and four glycopeptide subunits of protein molecular weight 36,600. The latter subunit has a total molecular weight of 51,700 when the carbohydrate is included. Attempts to dissociate this active enzyme complex to smaller size by increasing the detergent concentration led to inactivation. Thus, the smallest active particle in the presence of Lubrol WX contains the two polypeptide subunits in a mole ratio of 2:4 under conditions where the micellar form of detergent is present at a 70:1 molar ratio. This large excess of Lubrol WX eliminates any possibility of artificial togetherness as the result of statistical considerations.
The permeabilities of planar lipid bilayer (egg phosphatidylcholine-decane) membranes to butyric and formic acids were measured by tracer and pH electrode techniques . The purposes of the study were (a) to establish criteria for the applicability of each method and (b) to resolve a discrepancy between previously published permeabilities determined using the different was caused by unstirred layer effects in their poorly buffered solutions. The permeability to formic acid (pK. = 3 .75) measured by both tracer and pH electrode techniques was _ 10 -2 cm s -1 . However, if P' > P' l , the pH electrode technique cannot be used for measuring the permeabilities of weak acids with pK8's greater than^-4 .
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.