Although benthic infaunal communities are commonly measured to assess the effectiveness of environmental management in protecting biological resources, the tools used to interpret the resulting data are often subjective or site specific. We present an objective, quantitative index for application throughout the southern California coastal shelf environment that measures the condition of a benthic assemblage, with defined thresholds for levels of environmental disturbance. The index was calculated using a two-step process in which ordination analysis was employed to quantify a pollution gradient within a 717sample calibration data set. The pollution tolerance of each species was determined based upon its distribution of abundance along the gradient. The index is calculated as the abundance-weighted average pollution tolerance of species in a sample. Thresholds were established for reference condition as well as for four levels of biological response. Reference condition was established as the index value in samples taken distant from areas of anthropogenic activity and for which no contaminants exceeded the effects range low (ERL) screening levels. The four response levels were established as the index values at which key community attributes were lost. Independent data sets were used to validate the index in three ways. First, index sensitivity to a spatial gradient of exposure to a discharge from a point source was tested. Second, index response to a temporal gradient of exposure to a discharge from a point source was examined, testing index robustness to natural temporal variation. Third, the effect of changes in natural habitat (e.g., substrate, depth, and latitude) on index sensitivity was tested by evaluating the ability of the index to segregate samples taken in areas with high and low chemical exposure, across a gradient of physical habitats.

The exposure and uptake of environmental estrogenic compounds have been reported in previous studies of demersal flatfish species in the central Southern California Bight (SCB), USA. The objective of this study was to evaluate the estrogenic or feminizing activity of marine sediments from the SCB by using in vivo vitellogenin (VTG) assays in male or juvenile fish. In 2003, sediments were collected near wastewater outfalls serving the counties of Los Angeles (LACSD) and Orange (OCSD), and the city of San Diego (SD), California, USA. Cultured male California halibut (CH; Paralichthys californicus) were either directly exposed to sediments for 7 d or treated with two intraperitoneal injections of sediment extract over 7 d. The 17beta-estradiol (E2) equivalent values ranged from 1 to 90 microg/kg with LACSD > SD > OCSD. Measurable concentrations of E2 were observed in all sediment extracts and ranged from 0.16 to 0.45 ng/g. Estrone (El) was only observed in sediments near the LACSD outfall (0.6 ng/g). Alkylphenols and alkylphenol ethoxylates were observed in all sediment samples, but were highest near the OCSD outfall, where concentrations of nonylphenol were 3,200 ng/g. Fractionation studies of the LACSD sediment extract collected in 2004 failed to demonstrate relationships between VTG expression and 62 analytes, including E2, which was observed in the whole extract (2.9 ng/g). Oxybenzone (1.6 ng/g) was identified in bioactive fractions as well as unknown compounds of relatively high polarity. These results indicate that estrogen receptor-based assays may underestimate environmental estrogenic activity and estrogenic compounds other than classic natural and xenoestrogens may contribute to estrogenic activity of sediments from the SCB.

No abstract

No abstract

No abstract

No abstract

No abstract

No abstract