A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a crossdisciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoption of MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data. ' 2008 International Society for Advancement of Cytometry
Key termsimmunology; fluorescence-activated cell sorting; knowledge representation FLOW cytometry (FCM) systems have been available to investigators for over 30 years, and the field continues to advance at a rapid rate. FCM has been responsible for major progress in basic and clinical research by enabling the phenotypic and functional characterization of individual cells in a high-throughput manner. Advances in the technology now allow for automated, multiparametric analyses of thousands of samples per day (1). Each data set can consist of multidimensional descriptions of millions of individual cells, producing data similar in size and complexity to gene expression microarrays. Like the microarray field, the ability to collect FCM data is outpacing the computational means for data handling and analysis. Furthermore, the lack of reporting standardization limits collaboration, independent validation/refutation, and meta-analysis, and thus minimizes the value of the wealth
Eleven years of eelgrass Zostera marina seed additions conducted in a coastal bay system where Z. marina had not been reported since 1933 have resulted in rapid Z. marina expansion beyond the initially seeded plots. From 1999 through 2010, 37.8 million viable seeds were added to 369 individual plots ranging in size from 0.01 to 2 ha totaling 125.2 ha in 4 coastal bays. Subsequent expansion from these initial plots to approximately 1700 ha of bay bottom populated with Z. marina through 2010 is attributable to seed export from the original plots and subsequent generations of seedlings originating from those exports. Estimates of annual patch vegetative expansion showed mean estimated diameter increasing at varying rates from 10 to 36 cm yr −1 , consistent with rhizome elongation rates reported for Z. marina. Water quality data collected over 7 yr by spatially intensive sampling, as well as fixed-location continuous monitoring, document conditions in all 4 bays that are adequate to support Z. marina growth. In particular, median chlorophyll levels for the entire sampling period were between 5 and 6 µg l −1 for each of the bays, and median turbidity levels, while exhibiting seasonal differences, were between 8 and 9 NTU. The recovery of Z. marina initiated in this coastal bay system may be unique in seagrass recovery studies because of how the recovery was initiated (seeds rather than adult plants), how rapidly it occurred (years rather than decades), and the explicit demonstration of how one meadow modulated water clarity and altered sediments as it developed and expanded. Our results offer a new perspective on the role seeds can play in recovery dynamics at large spatial scales.
Eelgrass Zostera marina L. distribution patterns in the mid-Atlantic region of the USA have shown complex changes, with recovery from losses in the 1930s varying between the coastal lagoons and Chesapeake Bay. Restoration efforts in the coastal bays of Virginia introduced Z. marina back to this system, and expansion since 2005 has averaged 66% yr
To characterize further the nature of haemostatic impairment in haemorrhagic fever with renal syndrome, we assessed platelet function in 9 patients in whom the diagnosis was serologically confirmed. Defective platelet aggregation was demonstrated in every patient. An abnormality of the granule release reaction was demonstrated in all of 7 patients tested. Gel-filtered platelets from a normal subject showed normal aggregation in plasma from a patient with impaired aggregation, which is evidence for an intrinsic platelet defect, and against the presence of a circulating inhibitor in this patient.
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