The present study was designed to examine the influence of androgens on the size, morphological appearance, and fluid and protein secretory capacity of the lacrimal gland in a variety of species. For comparison, we also sought to determine whether other hormones might stimulate the growth and secretory ability of this tissue. Lacrimal glands and tears were obtained from intact, surgically-operated and/or hormone-treated rats, mice, guinea pigs and rabbits and the processed for histological and/or image analysis techniques. Our results demonstrated that: 1) androgens modulate the ultrastructural features of rat lacrimal tissue; 2) androgens may influence the weight, morphological aspects, and secretion of the lacrimal gland, however, these effects are not uniform in mice, rats, guinea pigs and rabbits, but instead are dependent upon the species and/or the animal's endocrine status; and 3) treatment with testosterone, prolactin, growth hormone, alpha-melanocyte stimulating hormone, insulin, or thyroxine plus dexamethasone had no impact on the absolute or relative size of the lacrimal gland, or the tear volume, of hypophysectomized male rats. Overall, these findings indicate that androgens may regulate the structure and secretion of the lacrimal gland. However, the nature and extent of this hormone action is species-dependent and may be modified by alterations in the endocrine environment.