ObjectiveThe purpose of the study is to investigate potential of antioxidant property of ethanolic root extract of Asparagus racemosus Linn (EEAR).MethodsIn vitro evaluation antioxidant property of EEAR was done using various methods like DPPH scavenging activity, hydroxyl radical scavenging activity, and nitric oxide scavenging activity. HPTLC fingerprint analysis was performed for qualitative determination of possible number of components from the ethanolic extract. Acute toxicity study was performed in Wistar rat and an OECD guideline 423 was followed.ResultsThe yield value was found 0.96% from EEAR. A concentration of 468.57 ± 3.002 μg/ml of probable antioxidant material from EEAR was required to scavenge 50% of DPPH. The IC50 value of EEAR were found to be 508.17 ± 7.37 μg and 416.57 ± 5.08 μg when determined by hydroxyl radical and nitric oxide scavenging assay respectively. The reducing powers of EEAR was 0.295 ± 0.0037 at 125 μg/ml and increased to 0.934 ± 0.0005 at 500 μg/ml. HPTLC fingerprint data supports several basic informations like isolation, purification, quality evaluation and standardization. No sign of toxicity was observed after treated with 2000 mg/kg of EEAR.ConclusionThe obtained data highlight the potential role of EEAR as a source of natural antioxidants.
This study investigates the preparation of colon specific drug delivery of diclofenac sodium (DS) using maltiparticulate system with chitosan phthalate (CP). Technique adopted to prepare sphere shaped multiparticulate system was extrusion and spheronization. The prepared pellets resulted with good spherical geometry with 1.1 ± 0.20 mm diameter. DS loaded system shows a maximum delivery pH of 7.4 and the same depressed at pH 1.2. The developed system significantly (p < .05) increase in the peak plasma concentration (C max ) of DS pellets when compared to that of pure DS. As expected the novel formulation showed a significant increase in oral bioavailability of DS for about three times. Additionally, the CP pellets of DS showed a momentous improvement in anti-inflammatory activity and reduced/ inhibited ulcer index.
K E Y W O R D Schitosan phthalate, in vivo, pH dependent, sustained release
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.