Isolation and enumeration of circulating tumor cells (CTCs) are used to monitor metastatic disease progression and guide cancer therapy. However, currently available technologies are limited to cells expressing specific cell surface markers, such as epithelial cell adhesion molecule (EpCAM) or have limited specificity because they are based on cell size alone. We developed a device, ApoStream TM that overcomes these limitations by exploiting differences in the biophysical characteristics between cancer cells and normal, healthy blood cells to capture CTCs using dielectrophoretic technology in a microfluidic flow chamber. Further, the system overcomes throughput limitations by operating in continuous mode for efficient isolation and enrichment of CTCs from blood. The performance of the device was optimized using a design of experiment approach for key operating parameters such as frequency, voltage and flow rates, and buffer formulations. Cell spiking studies were conducted using SKOV3 or MDA-MB-231 cell lines that have a high and low expression level of EpCAM, respectively, to demonstrate linearity and precision of recovery independent of EpCAM receptor levels. The average recovery of SKOV3 and MDA-MB-231 cancer cells spiked into approximately 12 Â 10 6 peripheral blood mononuclear cells obtained from 7.5 ml normal human donor blood was 75.4% 6 3.1% (n ¼ 12) and 71.2% 6 1.6% (n ¼ 6), respectively. The intra-day and inter-day precision coefficients of variation of the device were both less than 3%. Linear regression analysis yielded a correlation coefficient (R

In the tradition of research on political tolerance and democratic rights in context, this study uses a national survey of Americans conducted shortly after the September 11, 2001 attack on America to investigate people's willingness to trade off civil liberties for greater personal safety and security. We find that the greater people's sense of threat, the lower their support for civil liberties. This effect interacts, however, with trust in government. The lower people's trust in government, the less willing they are to trade off civil liberties for security, regardless of their level of threat. African Americans are much less willing to trade civil liberties for security than whites or Latinos, even with other factors taken into account. This may reflect their long-standing commitment to the struggle for rights. Liberals are less willing to trade off civil liberties than moderates or conservatives, but liberals converge toward the position taken by conservatives when their sense of the threat of terrorism is high. While not a forecast of the future, the results indicate that Americans' commitment to democratic values is highly contingent on other concerns and that the context of a large-scale threat to national or personal security can induce a substantial willingness to give up rights.

In the tradition of research on political tolerance and democratic rights in context, this study uses a national survey of Americans conducted shortly after the September 11, 2001 attack on America to investigate people's willingness to trade off civil liberties for greater personal safety and security. We find that the greater people's sense of threat, the lower their support for civil liberties. This effect interacts, however, with trust in government. The lower people's trust in government, the less willing they are to trade off civil liberties for security, regardless of their level of threat. African Americans are much less willing to trade civil liberties for security than whites or Latinos, even with other factors taken into account. This may reflect their long-standing commitment to the struggle for rights. Liberals are less willing to trade off civil liberties than moderates or conservatives, but liberals converge toward the position taken by conservatives when their sense of the threat of terrorism is high. While not a forecast of the future, the results indicate that Americans' commitment to democratic values is highly contingent on other concerns and that the context of a large-scale threat to national or personal security can induce a substantial willingness to give up rights.

rh-Endo was safe and well tolerated. rh-Endo pharmacokinetic profiles achieved area under the concentration-time curves associated with activity in preclinical models. Evidence of minor antitumor activity was observed and further studies are indicated.