Recanzone, Gregg H., Darren C. Guard, and Mimi L. Phan. Frequency and intensity response properties of single neurons in the auditory cortex of the behaving macaque monkey. J. Neurophysiol. 83: 2315Neurophysiol. 83: -2331Neurophysiol. 83: , 2000. Response properties of auditory cortical neurons measured in anesthetized preparations have provided important information on the physiological differences between neurons in different auditory cortical areas. Studies in the awake animal, however, have been much less common, and the physiological differences noted may reflect differences in the influence of anesthetics on neurons in different cortical areas. Because the behaving monkey is gaining popularity as an animal model in studies exploring auditory cortical function, it has become critical to physiologically define the response properties of auditory cortical neurons in this preparation. This study documents the response properties of single cortical neurons in the primary and surrounding auditory cortical fields in monkeys performing an auditory discrimination task. We found that neurons with the shortest latencies were located in the primary auditory cortex (AI). Neurons in the rostral field had the longest latencies and the narrowest intensity and frequency tuning, neurons in the caudomedial field had the broadest frequency tuning, and neurons in the lateral field had the most monotonic rate/level functions of the four cortical areas studied. These trends were revealed by comparing response properties across the population of studied neurons, but there was considerable variability between neurons for each response parameter other than characteristic frequency (CF) in each cortical area. Although the neuronal CFs showed a systematic spatial organization across AI, no such systematic organization was apparent for any other response property in AI or the adjacent cortical areas. The results of this study indicate that there are physiological differences between auditory cortical fields in the behaving monkey consistent with previous studies in the anesthetized animal and provide insights into the functional role of these cortical areas in processing acoustic information. I N T R O D U C T I O NStudies in the macaque monkey auditory cortex have defined several cortical fields based on physiological, anatomic, and combined techniques

Lesion studies have indicated that the auditory cortex is crucial for the perception of acoustic space, yet it remains unclear how these neurons participate in this perception. To investigate this, we studied the responses of single neurons in the primary auditory cortex (AI) and the caudomedial field (CM) of two monkeys while they performed a sound-localization task. Regression analysis indicated that the responses of approximately 80% of neurons in both cortical areas were significantly correlated with the azimuth or elevation of the stimulus, or both, which we term "spatially sensitive." The proportion of spatially sensitive neurons was greater for stimulus azimuth compared with stimulus elevation, and elevation sensitivity was primarily restricted to neurons that were tested using stimuli that the monkeys also could localize in elevation. Most neurons responded best to contralateral speaker locations, but we also encountered neurons that responded best to ipsilateral locations and neurons that had their greatest responses restricted to a circumscribed region within the central 60 degrees of frontal space. Comparing the spatially sensitive neurons with those that were not spatially sensitive indicated that these two populations could not be distinguished based on either the firing rate, the rate/level functions, or on their topographic location within AI. Direct comparisons between the responses of individual neurons and the behaviorally measured sound-localization ability indicated that proportionally more neurons in CM had spatial sensitivity that was consistent with the behavioral performance compared with AI neurons. Pooling the responses across neurons strengthened the relationship between the neuronal and psychophysical data and indicated that the responses pooled across relatively few CM neurons contain enough information to account for sound-localization ability. These data support the hypothesis that auditory space is processed in a serial manner from AI to CM in the primate cerebral cortex.

Sound localization ability has traditionally been studied using either a relative localization task, where thresholds to determine a difference in sound source location is approximately 1-10 degrees, or an absolute localization task, where the range of estimates of the source of a sound are 4-30 degrees. In order to directly relate these two psychophysical methods, we compared the psychometric functions from a relative localization task in a human subject to the same subject's performance on an absolute localization task using three different acoustic stimuli: Gaussian noise, 1-kHz tones, and 4-kHz tones. The results showed that the relative localization threshold was a poor indicator of the range of estimates of the same stimulus in absolute space, however, the width of the relative localization psychometric functions was well correlated with the width of the distribution of estimates made in the absolute localization task. It is concluded that the relative localization psychometric functions, but not threshold, provides a reliable estimate of absolute spatial localization ability in human subjects, and suggested that the same neuronal mechanisms can underlie the psychophysical data using both methods.