Zika virus (ZIKV) infection is now firmly linked to congenital Zika syndrome (CZS), including fetal microcephaly. While Aedes species of mosquito are the primary vector for ZIKV, sexual transmission of ZIKV is a significant route of infection. ZIKV has been documented in human, mouse, and nonhuman primate (NHP) semen. It is critical to establish NHP models of the vertical transfer of ZIKV that recapitulate human pathogenesis. We hypothesized that vaginal deposition of ZIKV-infected baboon semen would lead to maternal infection and vertical transfer in the olive baboon (Papio anubis). Epidemiological studies suggest an increased rate of CZS in the Americas compared to the original link to CZS in French Polynesia; therefore, we also compared the French Polynesian (FP) ZIKV isolate to the Puerto Rican (PR) isolate. Timed-pregnant baboons (n = 6) were inoculated via vaginal deposition of baboon semen containing 106 focus-forming units (FFU) of ZIKV (n = 3 for FP isolate H/PF/2013; n = 3 for PR isolate PRVABC59) at midgestation (86 to 95 days of gestation [dG]; term, 183 dG) on day 0 (all dams) and then at 7-day intervals through 3 weeks. Maternal blood, saliva, and cervicovaginal wash (CVW) samples were obtained. Animals were euthanized at 28 days (n = 5) or 39 days (n = 1) after the initial inoculation, and maternal/fetal tissues were collected. Viremia was achieved in 3/3 FP ZIKV-infected dams and 2/3 PR ZIKV-infected dams. ZIKV RNA was detected in CVW samples of 5/6 dams. ZIKV RNA was detected in lymph nodes but not the ovaries, uterus, cervix, or vagina in FP isolate-infected dams. ZIKV RNA was detected in lymph nodes (3/3), uterus (2/3), and vagina (2/3) in PR isolate-infected dams. Placenta, amniotic fluid, and fetal tissues were ZIKV RNA negative in the FP isolate-infected dams, whereas 2/3 PR isolate-infected dam placentas were ZIKV RNA positive. We conclude that ZIKV-infected semen is a means of ZIKV transmission during pregnancy in primates. The PR isolate appeared more capable of widespread dissemination to tissues, including reproductive tissues and placenta, than the FP isolate. IMPORTANCE Zika virus remains a worldwide health threat, with outbreaks still occurring in the Americas. While mosquitos are the primary vector for the spread of the virus, sexual transmission of Zika virus is also a significant means of infection, especially in terms of passage from an infected to an uninfected partner. While sexual transmission has been documented in humans, and male-to-female transmission has been reported in mice, ours is the first study in nonhuman primates to demonstrate infection via vaginal deposition of Zika virus-infected semen. The latter is important since a recent publication indicated that human semen inhibited, in a laboratory setting, Zika virus infection of reproductive tissues. We also found that compared to the French Polynesian isolate, the Puerto Rican Zika virus isolate led to greater spread throughout the body, particularly in reproductive tissues. The American isolates of Zika virus appear to have acquired mutations that increase their efficacy.

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To determine whether the persistent presence of antibodies to recombinant antigens of the hepatitis C virus (HCV) corresponds to the presence of hepatitis C virus RNA in the same serum, 85 anti-HCV positive patients were studied by the polymerase chain reaction (PCR). The focus of the research was on patients with chronic hepatitis. Eighty- three patients were found to be positive by PCR; only two were negative. In addition, liver biopsies taken from seven patients positive for anti-HCV were shown to contain HCV-specific RNA. Sera collected from three patients suspected to have NANB hepatitis on the basis of clinical symptoms were negative both for HCV antibodies and HCV RNA. The correlation between HCV antibody positivity and detection of HCV RNA was 97.6%.

44 45 ZIKV infection is associated with pregnancy loss, fetal microcephaly and other 46 malformations. While Aedes sp. of mosquito are the primary vector for ZIKV, sexual 47 transmission of ZIKV is a significant route of infection. ZIKV has been documented in 48 human, mouse and non-human primate (NHP) semen. It is critical to establish NHP 49 models of vertical transfer of ZIKV that recapitulate human ZIKV pathogenesis. We 50 hypothesized that vaginal deposition of ZIKV infected baboon semen would lead to 51 maternal infection and vertical transfer in the olive baboon (Papio anubis). Timed 52 pregnant baboons (n=6) were inoculated via vaginal deposition of baboon semen 53 containing 10 6 ffu ZIKV (n=3, French Polynesian isolate:H/PF/2013, n=3 Puerto Rican 54 isolate:PRVABC59) at mid-gestation (86-95 days gestation [dG]; term 183dG) on day (d) 550 (all dams), and then at 7 day intervals through three weeks. Maternal blood, saliva and 56 cervico-vaginal washes were obtained at select days post-inoculation. Animals were 57 euthanized at 28 days post initial inoculation (dpi; n=5) or 39 dpi (n=1) and maternal/fetal 58 tissues collected. vRNA was quantified by qPCR. Viremia was achieved in 3/3 FP ZIKV 59 infected dams and 2/3 PR ZIKV. ZIKV RNA was detected in cvw (5/6 dams;). ZIKV RNA 60 was detected in lymph nodes, but not ovary, uterus, cervix or vagina in the FP ZIKV 61 dams but was detected in uterus, vagina and lymph nodes. Placenta, amniotic fluid and 62 all fetal tissues were ZIKV RNA negative in the FP infected dams whereas 2/3 PR 63 infected dam placentas were ZIKV RNA positive. We conclude that ZIKV infected 64 semen is a means of ZIKV transmission during pregnancy in primates. The PR isolate 65 appeared more capable of wide spread dissemination to tissues, including placenta 66 compared to the FP strain. 67 68 69 IMPORTANCE 70 Due to its established link to pregnancy loss, microcephaly and other major congenital 71 anomalies, Zika virus (ZIKV) remains a worldwide health threat. Although mosquitoes 72 are the primary means of ZIVK transmission, sexual transmission in human populations 73 is well documented and provides a means for widespread dissemination of the virus. 74 Differences in viremia, tissue distribution, immune responses and pregnancy outcome 75 from sexually transmitted ZIKV compared to the subcutaneous route of infection are 76 needed to better clinically manage ZIKV in pregnancy. Through our previous work, we 77 have developed the olive baboon as a non-human primate model of ZIKV infection that 78 is permissible to ZIKV infection via the subcutaneous route of inoculation and transfer of 79 ZIKV to the fetus in pregnancy. The current study evaluated the course of ZIKV infection 80 after vaginal inoculation of ZIKV in pregnant baboons at mid-gestation using baboon 81 semen as the carrier and comparing two isolates of ZIKV, the French Polynesian isolate 82 first associated with microcephaly and the Puerto Rican isolate, associated with an 83 increased risk of microcephaly observed in the America...

OBJECTIVE: Mitochondria are cellular organelles that are required for ATP production, metabolism, and calcium homeostasis, and play a key role in oocyte and embryo development. In this study we aimed to determine whether metabolic imaging using FLIM to detect autofluorescence of NADH and FAD+ (key components of oxidative phosphorylation) can identify metabolic differences between normal oocytes and those with metabolic dysfunction.DESIGN: Experimental study. MATERIALS AND METHODS: Oocytes from old mice (1-year old) (n¼21) were compared to oocytes from young (12-week old) (n¼37) mice as a model of mild oocyte dysfunction. Oocytes obtained from mice with global knockout of Clpp (n¼55) were compared to wild type (WT) oocytes (n¼52) as a model of severe oocyte dysfunction. CLPP (caseinolytic peptidase P) mediates mitochondrial unfolded protein response (mt-UPR) and helps maintain homeostasis in response to metabolic and cellular stress. CLPP is required for oocyte and early embryo development, and ClpP-knockout results in female infertility and accelerated reproductive aging. Cumulus oophorus complexes (COCs) were collected 48h after injection with 5IU pregnant mare serum gonadotropin (PMSG, Sigma), and germinal vesicle (GV) oocytes were isolated. Fluorescence lifetime imaging microscopy (FLIM) was used to measure the naturally occurring autofluorescence of NADH and FAD+ in individual oocytes. A total of 8 metabolic parameters were obtained from each measurement (4 for each fluorophore) that are sensitive to differences in metabolic state: short (T1) and long (T2) fluorescence lifetime, fluorescence brightness (IRR), and fraction of the molecule bound to enzyme (FB). Matlab was used for statistical analysis.RESULTS: In older oocytes compared to young ones, FAD+ short fluorescence time (T1) was longer (p<0.001) and fluorescence brightness (IRR) was lower (p<0.01), while NADH short and long fluorescence times (T1 and T2) were shorter (p<0.0001 for both), and brightness (IRR) and fraction bound (FB) were lower (p<0.0001 and p<0.05, respectively). In ClpP-knockout oocytes compared to WT, FAD+ short and long fluorescence times (T1 and T2) were longer (p<0.0001) and brightness (IRR) was higher (p<0.01), while NADH long fluorescence time (T2) was longer (p<0.0001), and fraction bound (FB) was smaller (p<0.0001).CONCLUSIONS: In this study we used metabolic imaging to characterize the metabolic state of oocytes with mild (old) and severe (ClpP-knockout) e52

Zika virus (ZIKV) infection in pregnancy can produce catastrophic teratogenic damage to the developing fetus including microcephaly and congenital Zika syndrome (CZS). We previously described fetal CNS pathology occurring by three weeks post-ZIKV infection in Olive baboons at mid-gestation, including neuroinflammation, loss of radial glia (RG), RG fibers, neuroprogenitor cells (NPCs) resulting in disrupted NPC migration. In the present study, we explored fetal brain pathologies at term gestation resulting from ZIKV infection during either first or second trimester in the Olive baboon. In all dams, viremia resolved after 7 days post infection (dpi). One first trimester infected dam aborted at 5 dpi. All dams developed IgM and IgG response to ZIKV with ZIKV IgG detected in fetal serum. Substantial placental pathology and inflammation was observed including disruption of syncytiotrophoblast layers, delayed villous maturation, partially or fully thrombosed vessels, calcium mineralization and fibrin deposits. In the uterus, ZIKV was detected in ¾ first trimester but not in second trimester infected dams. While ZIKV was not detected in any fetal tissue at term, all fetuses exhibited varying degrees of neuropathology. Fetal brains from ZIKV infected dams exhibited a range of gross brain pathologies including irregularities of the major gyri and sulci of the cerebral cortex and cerebellar pathology. Frontal cortices of ZIKV fetuses showed a general disorganization of the six-layered cortex with degree of disorganization varying among the fetuses from the two groups. Frontal cortices from first but not second trimester infections exhibited increased microglia and astrocyte numbers (white matter) in both trimester infections. In the cerebellum, increased microglia were observed in both first and second trimester infected fetuses and decreased oligodendrocyte precursor cell populations in the cerebellar white matter in first trimester infections. In general, our observations are in accordance with those described in human ZIKV infected fetuses.

Zika virus (ZIKV) infection in pregnancy can produce catastrophic teratogenic damage to the developing fetus including microcephaly and congenital Zika syndrome (CZS). We previously described fetal CNS pathology occurring by three weeks post-ZIKV inoculation in Olive baboons at mid-gestation, including neuroinflammation, loss of radial glia (RG), RG fibers, neuroprogenitor cells (NPCs) resulting in disrupted NPC migration. In the present study, we explored fetal brain pathologies at term gestation resulting from ZIKV exposure during either first or second trimester in the Olive baboon. In all dams, vRNA in whole blood resolved after 7 days post inoculation (dpi). One first trimester infected dam aborted at 5 dpi. All dams developed IgM and IgG response to ZIKV with ZIKV IgG detected in fetal serum. Placental pathology and inflammation were observed including disruption of syncytiotrophoblast layers, delayed villous maturation, partially or fully thrombosed vessels, calcium mineralization and fibrin deposits. In the uterus, ZIKV was detected in ¾ first trimester but not in second trimester infected dams. While ZIKV was not detected in any fetal tissue at term, all fetuses exhibited varying degrees of neuropathology. Fetal brains from ZIKV inoculated dams exhibited a range of gross brain pathologies including irregularities of the major gyri and sulci of the cerebral cortex and cerebellar pathology. Frontal cortices of ZIKV fetuses showed a general disorganization of the six-layered cortex with degree of disorganization varying among the fetuses from the two groups. Frontal cortices from ZIKV inoculation in the first but not second trimester exhibited increased microglia, and in both trimester ZIKV inoculation, increased astrocyte numbers (white matter). In the cerebellum, increased microglia were observed in fetuses from both first and second trimester inoculation. In first trimester ZIKV inoculation, decreased oligodendrocyte precursor cell populations were observed in fetal cerebellar white matter. In general, our observations are in accordance with those described in human ZIKV infected fetuses.

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