BackgroundOsteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA.MethodsForty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction.ResultsExpression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group).ConclusionsThese findings may contribute to disease prevention and the development of therapeutic targets for OA.
Introduction: Osteoarthritis (OA) is a widely prevalent joint disease leading to motor disability and pain. Appropriate indicators for identifying patients at risk for this progressive disease, identifying molecular events for detecting early phases of the disease, or biomarkers to screen for treatment responses, however, are lacking. Micro RNAs (miRNAs), which play crucial roles in OA, could be potential biomarkers of OA. Because circulating miRNA levels reflect the disease state, they may be useful for OA screening and as diagnostic tools, reducing the need for invasive procedures and minimizing the cost of current diagnostic methods. Materials and methods: The expression levels of 18 microRNAs (let-7e-5p, miR-21-5p, miR-93-5p, miR-101-3p, miR-103a-3p, miR-130a-3p miR-146a-5p, miR-16-5p, miR-193b-3p miR-199a-3p, miR-210-3p, miR-222-3p, miR-22-3p, miR-27a-3p, miR-27b-3p, miR-335-5p, miR-454-3p, and miR-98-5p) were analyzed by quantitative real-time polymerase chain reaction in the cartilage tissues and serum samples of 28 OA patients and were compared to those of 2 healthy controls. Results: Expression of microRNA-146a-5p was significantly upregulated in the cartilage (p=0.006) and serum (p=0.002) of OA patients. The expression levels of miR-146a-5p in the serum were positively correlated with those in the cartilage (Pearson correlation coefficient R=0.32; p=0.002). Conclusion: miR-146a-5p was significantly overexpressed in patients with OA, both in the articular cartilage tissue and serum, with a positive correlation between the levels in both types of samples. Therefore, the miR-146a-5p serum level could reflect the molecular processes in the cartilage, suggesting its clinical utility as a biomarker for OA management. Implementing noninvasive biomarker using serum miRNAs involves the analysis of the misregulated miRNAs linked to the cartilage pathology.
ErratumIn the original publication of this article [1], was an error in the abstract and the methods section which was published with an incorrect kit citation.The error 1: Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit.Should be read instead: Total RNA (containing miRNA species) from cartilage was isolated using a miRCURYTM Isolation Kit.The error 2: For miRNA profiling, total RNA was purified and prepared. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit (Exiqon, Vedbaek, Denmark) following the manufacturer's suggested protocol.Should be read instead: For miRNA profiling, total RNA was purified and prepared. Total RNA (containing miRNA species) from cartilage was isolated using a miRCURYTM Isolation Kit (Exiqon, Vedbaek, Denmark) following the manufacturer's suggested protocol.
Introduction: Osteoarthritis (OA) is the most common form of arthritis that affects millions of people worldwide. Despite advances in medicine, OA still remains incurable. Scientists are still seeking candidates for biomarker-based risk stratification and the early detection of disease. Measurement of miRNAs in the serum may become a powerful tool in the development of diagnostic biomarker. Purpose: The aim of this study was to examine the expression profiles of 20 miRNAs in the serum of patients with OA. Methods: We used RT-qPCR method to measure the expression profile of selected miRNAs. Results: Six miRNAs showed differential expression between OA and normal serum samples. miR-146a-5p and miR-98-5p were significantly upregulated (P < 0.05), while miR-222-3p, miR-22-3p, miR-27a-3p and miR-93-5p were downregulated (P < 0.05) compared to the control group. To the best of our knowledge, this is the first work that shows that expression of these six miRNAs is different between serum OA samples and 13 healthy controls. However, we did not find any statistically significant relationship between the miRNAs expression and clinical characteristics. Conclusions: Our findings suggest that miRNAs can be used as potential biomarkers for OA, but further research is needed to assess the usefulness of serum miRNAs in miRNA-based prognostic and therapeutic approaches.
Introduction: Osteoarthritis (OA) is the most common form of arthritis that affects millions of people worldwide. Despite advances in medicine, OA still remains incurable.Scientists are still seeking candidates for biomarker-based risk stratification and the early detection of disease. Measurement of miRNAs in the serum may become a powerful tool in the development of diagnostic biomarker.Purpose: The aim of this study was to examine the expression profiles of 20 miRNAs in the serum of patients with OA. Methods:We used RT-qPCR method to measure the expression profile of selected miRNAs.Results: Six miRNAs showed differential expression between OA and normal serum samples. miR-146a-5p and miR-98-5p were significantly upregulated (P < 0.05), while miR-222-3p, miR-22-3p, miR-27a-3p and miR-93-5p were downregulated (P < 0.05) compared to the control group. To the best of our knowledge, this is the first work that shows that expression of these six miRNAs is different between serum OA samples and 13 healthy controls. However, we did not find any statistically significant relationship between the miRNAs expression and clinical characteristics. Conclusions:Our findings suggest that miRNAs can be used as potential biomarkers for OA, but further research is needed to assess the usefulness of serum miRNAs in miRNA-based prognostic and therapeutic approaches.
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