Tremendous interest in research of small extracellular vesicles (sEVs) is driven by the participation of vesicles in a number of biological processes in the human body. Being released by almost all cells of the body, sEVs present in complex bodily fluids form the so-called intercellular communication network. The isolation and profiling of individual fractions of sEVs secreted by pathological cells are significant in revealing their physiological functions and clinical importance. Traditional methods for isolation and purification of sEVs from bodily fluids are facing a number of challenges, such as low yield, presence of contaminants, long-term operation and high costs, which restrict their routine practical applications. Methods providing a high yield of sEVs with a low content of impurities are actively developing. Bead-assisted platforms are very effective for trapping sEVs with high recovery yield and sufficient purity for further molecular profiling. Here, we review recent advances in the enrichment of sEVs via bead-assisted platforms emphasizing the type of binding sEVs to the bead surface, sort of capture and target ligands and isolation performance. Further, we discuss integration-based technologies for the capture and detection of sEVs as well as future research directions in this field.
This paper analyzes the essence of the phenomenological method as it is used in certain theories in ethics and legal philosophy. The purpose of the paper is to provide a full study of phenomenology to determine its place in modern philosophical thought. The paper used methods of the history of philosophy, especially method of rational reconstruction, and based on interpretation of the classical phenomenological texts (E. Husserl, E. Levinas, A. Reinach). The main result of the paper is the justification that the unity of logic, ontology and ethics became the ground of application of the phenomenological method in the field of legal and ethical knowledge. Therefore the ideas of E. Levinas’s ethical phenomenology were the basis for understanding ethics as the “first philosophy” in a phenomenological context. The main conclusion of this paper is that the ethical dimension of responsibility for the actions of the subject and their consequences expands the horizons of phenomenological reduction and allows us to reveal the essence of legal reality in a new way. The paper was carried out within the framework of the HSE research project “Ethics and Law: correlation and mechanisms of mutual influence”.
The purpose of the article is to conduct a historical and philosophical study of the methodological foundations of the implementation of the phenomenological method in the philosophy of law of the XX century on the example of the legal concepts of A. Reinakh and N.N. Alekseeva developed within the framework of continental tradition. The author defends the thesis about the methodological potential of the phenomenological method, the need to expand the research tools of legal science.
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