SignificanceAntibiotics have been widely used to treat bacterial infections and are also found in the environment. Bacteria have evolved various resistance mechanisms, allowing them to overcome antibiotic exposure and raising important health issues. Here, we report a bacterial antibiotic resistance mechanism, based on ribosome splitting and recycling, ensuring efficient translation even in presence of lincomycin and erythromycin, two antibiotics that block protein synthesis. This mechanism is mediated by a HflX-like protein, encoded by lmo0762 in Listeria monocytogenes, whose expression is tightly regulated by a transcriptional attenuation mechanism. This gene increases bacterial fitness in the environment. Our results raise the possibility that other antibiotic-induced resistance mechanisms remain to be discovered.

Microbial populations and communities are heterogeneous, yet capturing their diverse activities has proven challenging at the relevant spatiotemporal scales. Here we present par-seqFISH, a targeted transcriptome-imaging approach that records both gene-expression and spatial context within microscale assemblies at a single-cell and molecule resolution. We apply this approach to the opportunistic bacterial pathogen, Pseudomonas aeruginosa, analyzing ~600,000 individuals across dozens of physiological conditions in planktonic and biofilm cultures. We explore the phenotypic landscape of this bacterium and identify metabolic and virulence related cell-states that emerge dynamically during growth. We chart the spatial context of biofilm-related processes including motility and kin-exclusion mechanisms and identify extensive and highly spatially-resolved metabolic heterogeneity. We find that distinct physiological states can co-exist within the same biofilm, just a few microns away, underscoring the importance of the microenvironment. Together, our results illustrate the complexity of microbial populations and present a new way of studying them at high-resolution.

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14One of the most frequent injury patterns leading to early death of polytraumatized 15 patients is hemorrhagic shock combined with traumatic brain injury. Currently, there 16 33 ml/kg of HTS or WB. Taken together, resuscitation with WB at 9-18ml/kg or HTS at 34 9ml/kg is optimal for treatment of combined injury. 3 35