& TLC-densitometric method was developed for determination of danofloxacin (DAN) in presence of its photodegradation products. Silica gel HPTLC Lichrospher 60 F 254 plates were used as the stationary phase and methanol:acetone:1 M citric acid:triethylamine (2.8:2:0.2:0.5, v=v=v=v) as the mobile phase. The elaborated method meets the acceptance criteria for specificity, linearity, sensitivity, accuracy, and precision. The linear regression analysis for the calibration curve showed a good linear correlation over the concentration range 1.25-3.75 lg per band, with determination coefficient, exceeding 0.9806. Accuracy of the method expressed as % recovery at three concentration levels was from 95.56% to 97.30%. Good precision and intermediate precision with % RSD less than 0.92% was also observed. The limits of detection and quantification were respectively 1.10 and 3.40 ng per band. Satisfactory results of validation of the method were also confirmed by determination of DAN in veterinary commercial preparation. The photodegradation process of DAN followed kinetics of the first order reaction for the substrate.
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