The complete mitochondrial genome of the microalgae
Dunaliella salina
strain SQ (GenBank accession number: KX641170) was
de novo
assembled and annotated using Illumina MiSeq sequencing data. The mitochondrial genome is 41,904 bp long with 31.85% GC content and contains 7 protein-coding genes, 16 introns, 3 ribosomal RNA genes and 3 transfer RNA genes. To date, only two complete mitochondrial genomes of
Dunaliella salina
strains have been reported, and this genome provides knowledge to the study of genetic variations and evolution of mitochondrial genomes of
Dunaliella salina
strains.
Marine sediments harbor an outstanding level of microbial diversity supporting diverse metabolic activities. Sediments in the Gulf of Mexico (GoM) are subjected to anthropic stressors including oil pollution with potential effects on microbial community structure and function that impact biogeochemical cycling. We used metagenomic analyses to provide significant insight into the potential metabolic capacity of the microbial community in Southern GoM deep sediments. We identified genes for hydrocarbon, nitrogen and sulfur metabolism mostly affiliated with Alpha and Betaproteobacteria, Acidobacteria, Chloroflexi and Firmicutes, in relation to the use of alternative carbon and energy sources to thrive under limiting growth conditions, and metabolic strategies to cope with environmental stressors. In addition, results show amino acids metabolism could be associated with sulfur metabolism carried out by Acidobacteria, Chloroflexi and Firmicutes, and may play a crucial role as a central carbon source to favor bacterial growth. We identified the tricarboxylic acid cycle (TCA) and aspartate, glutamate, glyoxylate and leucine degradation pathways, as part of the core carbon metabolism across samples. Further, microbial communities from the continental slope and abyssal plain show differential metabolic capacities to cope with environmental stressors such as oxidative stress and carbon limiting growth conditions, respectively. This research combined taxonomic and functional information of the microbial community from Southern GoM sediments to provide fundamental knowledge that links the prokaryotic structure to its potential function and which can be used as a baseline for future studies to model microbial community responses to environmental perturbations, as well as to develop more accurate mitigation and conservation strategies.
The complete chloroplast genome of the microalgae Dunaliella salina strain SQ was determined in this study. The total length of the chloroplast genome is 243,635 bp with 29.73% GC content. The genome is composed by a small single copy (SSC) region of 101,527 bp and a large single-copy region of 107,815 bp separated by two inverted repeats (IR) regions of 17,145 bp. A total of 98 genes were annotated, including 66 coding genes, 3 rRNAs, and 29 tRNAs. This complete plastid genome can be used to elucidate genetic variations in organellar genomes between D. salina strains.
Host genetics and diet can exert an influence on microbiota and, therefore, on feeding efficiency. This study evaluated the effect of genetic line (fast-growth and high-resistance) in Pacific white shrimp (Litopenaeus vannamei) on the hepatopancreatic microbiota and its association with the feeding efficiency in shrimp fed with diets containing different protein sources. Shrimp (2.08 ± 0.06 g) from each genetic line were fed for 36 days with two dietary treatments (animal and vegetable protein). Each of the four groups was sampled, and the hepatopancreatic metagenome was amplified using specific primers for the variable V4 region of the 16S rRNA gene. The PCR product was sequenced on the MiSeq platform. Nineteen bacterial phyla were detected, of which Proteobacteria was the most abundant (51.0-72.5 %), Bacteroidetes (3.6-23.3 %), Firmicutes (4.2-13.7 %), Actinobacteria (1.9-12.1 %), and Planctomycetes (1.3-9.5 %). Diet was the most influential factor in the taxonomic composition of the microbiota, while genetic line was not a strong influential factor. The results suggest that the taxonomic profile of the bacteria colonizing shrimp hepatopancreas was determined by the diet consumed, similar to what occurs in the intestine. Shrimp in the fast-growth line had greater feeding efficiency regardless of the diet supplied. Finally, the results suggest that Proteobacteria influenced (p < 0.05) the feeding efficiency of shrimp fed with a vegetable diet. Nevertheless, further studies are required to explore how shrimp genetic line-diet interaction influences microbiota for probiotic development and functional food formulation for farmed shrimp according to the genetic line.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.