These results suggest that low concentrations of T and DHT are associated with ultrastructural damage of the aortic endothelia in male rats.
Thrombospondin-2 (THBS2) is a secreted protein overexpressed in numerous cancers and may function as a diagnostic tumor marker. The objective of the present study was to investigate the diagnostic performance of serum THBS2 in early stage non-small-cell lung cancer (NSCLC). Serum THBS2 and Cyfra21-1 level were evaluated in blood samples of 112 patients from NSCLC groups and 51 healthy control (HC) groups. Receiver operator characteristic (ROC) curves were used to evaluate the diagnostic significance. Serum THBS2 level was significantly up-regulated in NSCLC patients compared with healthy control subjects (P<0.0001), and the postoperative THBS2 level decreased significantly (P<0.0001). ROC curves analysis demonstrated that THBS2 was a comparable biomarker as Cyfra21-1 to distinguish early stage NSCLC or lung squamous cell carcinoma (SC) from healthy control subjects. And Cyfra21-1 was observed with significantly improved performances by the combination of THBS2 to distinguish early stage NSCLC (P<0.05) as well as SC (P<0.05) from the control subjects. In addition, THBS2 was estimated to perform well in the diagnosis of patients with Cyfra21-1-negative NSCLC (area under the curve [AUC] = 0.73). In summary, the present study suggested that serum THBS2 might be an early diagnostic biomarker for NSCLC.
Many circRNAs are involved in the carcinogenesis of breast cancer (BCa) through the transcription of microRNAs (miRNAs) and mRNAs. This study investigated circRBM33 regulation of the miR-542-3p/hypoxia-inducible factor-1α (HIF-1α) axis in BCa. BCa clinical tissue samples were collected to test differential expressions of circRBM33, miR-542-3p, and HIF-1α. MCF-7 cells were subjected to normoxia or hypoxia and transfected with plasmids that regulated CircRBM33, miR-542-3p, and HIF-1α expression levels. Glycolysis was evaluated by measuring glucose consumption, lactic acid production, and protein expression of hexokinase 2, glucose transporter type 1 and lactic dehydrogenase A. Cell proliferation and apoptosis were also assessed, and the interactions between genes were explored. CircRBM33 and HIF-1α were upregulated, while miR-542-3p was downregulated in BCa tissue samples and cell lines. Hypoxia induced circRBM33 expression in BCa cells, which negatively regulated miR-542-3p expression. CircRBM33 knockdown or miR-542-3p rescue reduced glycolysis and proliferation and promoted apoptosis of BCa cells. MiR-542-3p inhibition rescued circRBM33 knockdown-mediated glycolysis, proliferation and apoptosis of BCa cells. MiR-542-3p targeted HIF-1α, and the overexpression of HIF-1α reversed the effect of miR-542-3p upregulation on glycolysis, proliferation, and apoptosis of BCa cells. Collectively, downregulating circRBM33 suppresses miR-542-3p-targeted HIF-1α expression, resulting in the inhibition of glycolysis and proliferation and the promotion of BCa cells’ apoptosis.
Objective: Diabetic foot (DF) is one of the common serious complications of diabetes, which is an important cause of death and disability, and is associated with diabetic vascular disease and diabetic neuropathy. The purpose of this study was to assess the incidence and risk factors for diabetic foot among a diabetic population. Methods: The study was a retrospective cohort review. The population studied was 348 male examinees of type 2 diabetes mellitus (DM), in which age-matched equal examinees (174 patients each) were with diabetic foot (DF Group) and with no indication of diabetic foot (non-DF group), hospitalized in the same year at The First Hospital of Jiaxing, China. Medical records were reviewed to collect clinical profile, including duration of disease, smoking, previous diabetic foot incidence, and medication (such as metformin). Categorical data between groups were analyzed using chi-square test (χ2). Unconditional logistic regression analysis was used for multi-factor analysis to identify the risk factors of diabetic foot. Results: Comparing the baseline data of DF Group and non-DF Group, age, course of disease, LDL-C and use of metformin showed no significant difference (P >0.05). The results of the logistic regression analysis showed that smoking history (OR=1.88, P=0.020), previous diabetic foot history (OR=2.290, P=0.016), Hcy (OR=1.194, p≤0.001) were independent risk factors for diabetic foot disease, and HgB (OR =0.984, P=0.021) was the protective factor of diabetic foot disease in male subjects. Age (OR=0.985, P=0.304), course of disease (OR=1.048, P=0.233), history of metformin use (OR=0.851, P=0.509), HbA1c (OR=1.302, P=0.059), LDL-C (OR=0.936, P=0.698), Creatinine (OR =1.010, P=0.326), and ALB (OR =0.943, P=0.084) were not significantly correlated with diabetic foot disease. Conclusions: Smoking, previous diabetic foot history, and homocysteine are independent risk factors for diabetic foot disease. HgB is the protective factor of diabetic foot disease in male patients.
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