Funding informationFonds Québécois de la recherche sur la nature et les technologies; Québec Ministry of Public Security Coastlines along the St. Lawrence Estuary and Gulf, Eastern Canada, are under increasing risk of flooding due to sea level rise and sea ice shrinking. Efficient and validated regional-scale coastal flood mapping approaches that include storm surges and waves are hence required to better prepare for the increased hazard. This paper compares and validates two different flood mapping methods: numerical flood simulations using XBeach and bathtub mapping based on total water levels, forced with multihazard scenarios of compound wave and water level events. XBeach is validated with hydrodynamic measurements. Simulations of a historical storm event are performed and validated against observed flood data over ã 25 km long coastline using multiple fit metrics. XBeach and the bathtub method correctly predict flooded areas (66 and 78%, respectively), but the latter overpredicts the flood extent by 36%. XBeach is a slightly more robust flood mapping approach with a fit of 51% against 48% for the bathtub maps. Deeper floodwater by~0.5 m is expected with the bathtub approach, and more buildings are vulnerable to a 100-year flood level. For coastal management at regional-scale, despite similar flood extents with both flood mapping approaches, results suggest that numerical simulati on with XBeach outperforms bathtub flood mapping.

SummaryThe microsporidian Encephalitozoon cuniculi is an obligate intracellular parasite that can cause opportunistic infections in AIDS patients. Spore invasion of host cells involves extrusion of a polar tube. After immunocytochemical identification of several polar tube proteins (PTPs) in E. cuniculi, a major PTP was isolated from two-dimensional gels and two peptide fragments were sequenced. The complete nucleotide sequence of the corresponding gene was obtained using a combination of PCR amplification and cloning techniques. The gene exists as a single copy per haploid genome and encodes an acidic proline-rich protein, with a deduced molecular mass of 37 kDa, that contains four tandemly arranged 26-amino-acid repeats. An N-terminal region of 22 residues represents a cleaved signal peptide, probably involved in the targeting of the PTP. No similarity with known proteins has been found. The protein was expressed in Escherichia coli, purified and injected into mice. The antisera reacted specifically with the polar tube in indirect immunofluorescence assays and electron microscope immunocytochemistry. Further identification of conserved and variable PTP structural motifs should be useful for diagnostic purposes and new therapeutic strategies.

On 6 December 2010, an extra-tropical storm reached Atlantic Canada, causing coastal flooding due to high water levels being driven toward the north shore of Chaleur Bay. The extent of flooding was identified in the field along the coastline at Maria using DGPS. Using the assumption that the maximum elevation of flooded areas represents the combination of astronomical tide, storm surge and wave runup, which is the maximum elevation reached by the breaking waves on the beach, all flood limits were identified. A flood-zone delineation was performed using GIS and LiDAR data. An empirical formula was used to estimate runup elevation during the flood event. A coastal flood map of the 6 December flood event was made using empirical data and runup calculations according to offshore wave climate simulations. Along the natural beach, results show that estimating runup based on offshore wave data and upper foreshore beach slope represents well the observed flood extent. Where a seawall occupies the beach, wave breaking occurs at the toe of the structure and wave height needs to be considered independently of runup. In both cases (artificial and natural), flood risk is underestimated if storm surge height alone is considered. There is a need to incorporate wave characteristics in order to adequately model potential flood extent. A coastal flooding projection is proposed for Pointe Verte based on total water levels estimated according to wave climate simulation return periods and relative sea-level rise for the Chaleur Bay.

Microsporidia can form small spores with a unique invasive apparatus featuring a long polar tube whose extrusion allows entry of infectious sporoplasm into a host cell. The reactivity of mouse polyclonal antibodies raised against sporal proteins from two microsporidian species belonging to different genera (Glugea atherinae and Encephalitozoon cuniculi) was studied by western blotting and indirect immunofluorescence. Whole protein antisera provided a few cross-reactions relatable to some proteins of the spore envelope or polar tube. Ultrastructural immunocytochemistry with murine antibodies against protein bands separated by sodium dodecylsulphate polyacrylamide gel electrophoresis allowed the assignment of several proteins to the polar tube (34, 75 and 170 kDa in Glugea, 35, 55 and 150 kDa in Encephalitozoon). Antigenic similarities were detected for the Glugea 34 kDa and Encephalitozoon 35 kDa polar tube proteins. Species-specific proteins were shown to be located in either the lamellar polaroplast of Glugea or the spore envelope of Encephalitozoon.