Despite the ubiquity and importance of microbial communities, understanding the population dynamics of mixed cultures in structured environments remains a fundamental problem in microbial ecology. Identifying bacterial strains within a complex microbial community represents a challenging technical problem. We describe a low-cost optomechanical device designed to acquire multi-channel timelapse images of bacterial colonies growing in agar plates. This device uses a system of addressable LEDs and fluorescence filters to estimate the spatio-temporal distribution of different fluorescently-tagged cells from time-lapse images obtained using a standard DSLR camera with a macro lens. We demonstrate the potential of this device with a range of applications from experimental microbiology.
Microbial ecosystems are composed of multiple species in constant metabolic exchange. A pervasive interaction in microbial communities is metabolic cross-feeding and occurs when the metabolic burden of producing costly metabolites is distributed between community members, in some cases for the benefit of all interacting partners. In particular, amino acid auxotrophies generate obligate metabolic inter-dependencies in mixed populations and have been shown to produce a dynamic profile of interaction that depends upon nutrient availability. However, identifying the key components that determine the pair-wise interaction profile remains a challenging problem, partly because metabolic exchange has consequences on multiple levels, from allocating proteomic resources at a cellular level to modulating the structure, function and stability of microbial communities. To evaluate how ppGpp-mediated resource allocation drives the population-level profile of interaction, here we postulate a multi-scale mathematical model that incorporates dynamics of proteome partition into a population dynamics model. We compare our computational results with experimental data obtained from co-cultures of auxotrophic Escherichia coli K12 strains under a range of amino acid concentrations and population structures. We conclude by arguing that the stringent response promotes cooperation by inhibiting the growth of fast-growing strains and promoting the synthesis of metabolites essential for other community members.
<abstract><p>Interactions between species are essential in ecosystems, but sometimes competition dominates over mutualism. The transition between mutualism-competition can have several implications and consequences, and it has hardly been studied in experimental settings. This work studies the mutualism between cross-feeding bacteria in strains that supply an essential amino acid for their mutualistic partner when both strains are exposed to antimicrobials. When the strains are free of antimicrobials, we found that, depending on the amount of amino acids freely available in the environment, the strains can exhibit extinction, mutualism, or competition. The availability of resources modulates the behavior of both species. When the strains are exposed to antimicrobials, the population dynamics depend on the proportion of bacteria resistant to the antimicrobial, finding that the extinction of both strains is eminent for low levels of the resource. In contrast, competition between both strains continues for high levels of the resource. An optimal control problem was then formulated to reduce the proportion of resistant bacteria, which showed that under cooperation, both strains (sensitive and resistant) are immediately controlled, while under competition, only the density of one of the strains is decreased. In contrast, its mutualist partner with control is increased. Finally, using our experimental data, we did parameters estimation in order to fit our mathematical model to the experimental data.</p></abstract>
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