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Materials, such as biopolymers, can be applied to produce scaffolds as mechanical support for cell growth in regenerative medicine. Two examples are polycaprolactone (PCL) and poly (lactic-coglycolic acid) (PLGA), both used in this study to evaluate the behavior of umbilical cord-derived mesenchymal stem cells. The scaffolds were produced by the 3D printing technique using PCL as a polymer covered with PLGA fibers obtained by electrospinning. The cells were seeded in three concentrations: 8.5×10 3 ; 25.5×10 3 and 51.0×10 3 on the two surfaces of the scaffolds. With scanning electron microscopy (SEM), it was observed that the electrospun fibers were integrated into the 3D printed matrices. Confocal laser scanning microscopy and SEM confirmed the presence of attached cells and the lactate dehydrogenase release test showed the scaffolds were not cytotoxic. The cells were able to differentiate into osteogenic and chondrogenic lineages on the scaffolds. Mechanical test showed that the cells seeded on the 3D printed PCL matrices coated with PLGA electrospun nanofibers (3D+ES+SC) did not show significant difference in tensile modulus than the pure PCL matrix (3D) or PCL matrices coated with PLGA electrospun nanofibers (3D+ES). The combination of the two polymers facilitated the production of a support with greater mechanical stability due to the presence of the 3D printed PCL matrices fabricated by melted filaments and greater cell adhesion due to the PLGA fibers. The scaffolds are suitable for use in cell therapy and also for tissue regeneration purposes.

Background: Little is known about the cognitive profile of Hereditary Spastic Paraplegias (HSP), where most scientific attention has been given to motor features related to corticospinal tract degeneration. Objectives: We aimed to perform a broad characterization of the cognitive functions of patients with pure and complicated HSP as well as to determine the frequency of abnormal cognitive performances in the studied subtypes. Methods: A two-center cross-sectional case-control study was performed. All individuals underwent cognitive assessment through screening tests (Mini Mental State Examination—MEEM and Montreal Cognitive Assessment—MOCA) and tests to assess specific cognitive functions (Verbal fluency with phonological restriction—FAS; Verbal categorical fluency—FAS-cat and Rey's Verbal Auditory Learning Test -RAVLT). Results: Fifty four patients with genetically confirmed HSP diagnosis, 36 with spastic paraplegia type 4 (SPG4), 5 SPG11, 4 SPG5, 4 cerebrotendinous xanthomatosis (CTX), 3 SPG7 and 2 SPG3A, and 10 healthy, unrelated control subjects, with similar age, sex, and education participated in the study. SPG4 patients had worse performances in MOCA, FAS, FAS-cat, and RAVLT when compared to controls. Most SPG4 patients presented cognitive changes not compatible with dementia, performing poorly in memory, attention and executive functions. SPG5 patients scored lower in executive functions and memory, and SPG7 patients performed poorly on memory tasks. All evaluated cognitive functions were markedly altered in CTX and SPG11 patients. Conclusions: Cognitive abnormalities are frequent in HSP, being more severe in complicated forms. However, cognitive impairments of pure HSPs might impact patients' lives, decreasing families' socioeconomic status and contributing to the overall disease burden.

In this study, an evaluation was performed to determine the in vitro bioactivity, viability of stem cells, and antibiofilm effect against Streptococcus mutans of two bioactive gel-glass 60SiO-36CaO-4PO (BG-A) and 80SiO-15CaO-5PO (BG-B) compositions. Both materials were bioactive and undergo the formation of hydroxycarbonate apatite (HCA) on their surfaces when immersed in simulated body fluid (SBF) after 12h, but the BG-A composition showed a more significant formation rate. The pH variation of the samples during the test in SBF indicated that an abrupt change had occurred for the BG-A composition within the first few hours, and the pH was subsequently maintained over time, supporting its stronger antibacterial effects against S. mutans. For the in vitro viability test using mesenchymal stem cells (MSCs), the BG-B showed significantly higher cell viability compared to the BG-A composition at concentrations of 0.125, 1.25 and 12.50mg/mL for 2days. These results indicated that the higher solubility of the BG-A glass favors bioactivity and antibacterial effects. However, as a result of rapid degradation, the increase in the concentration of ions in the cell culture medium was not favorable for cell proliferation. Thus, by varying the composition of glasses, and consequently their dissolution rate, it is possible to favor bioactivity, antimicrobial activity or stem cell proliferation for a particular application of interest.

Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is an early-onset, neurodegenerative disorder caused by mutations in SACS, firstly reported in Quebec, Canada. The disorder is typically characterized by childhood onset ataxia, spasticity, neuropathy and retinal hypermyelination. The clinical picture of patients born outside Quebec, however, is often atypical. In the present article, the authors describe clinical and neuroradiological findings that raised the suspicion of an ARSACS diagnosis in two female cousins with Germanic background from Rio Grande do Sul, Brazil. We present a review on the neuroimaging, ophthalmologic and neurophysiologic clues for ARSACS diagnosis. The early-onset, slowly progressive, spastic-ataxia phenotype of reported patients was similar to ARSACS patients from Quebec. The SACS sequencing revealed the novel homozygous c.5150_5151insA frameshift mutation confirming the ARSACS diagnosis. ARSACS is a frequent cause of early onset ataxia/spastic-ataxia worldwide, with unknown frequency in Brazil.

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Introduction:In the first months of 2018, there was an increase in the number of cases of fever possibly related to toxoplasmosis in the city of Santa Maria, Brazil, reaching significant values. Toxoplasmosis is an autoimmune acute infection usually asymptomatic in 80-90% of immunocompetent adults. In this outbreak, the intensity of the symptoms presented warrants attention.Objective:To report cases of the toxoplasmosis outbreak in the city of Santa Maria, Brazil.Methods:This is a cross-sectional study using data on the outbreak of toxoplasmosis in Santa Maria published in bulletins by the Municipal Health Department of Santa Maria, Rio Grande do Sul, Brazil.Results:The outbreak of toxoplasmosis in Santa Maria was confirmed on April 19, 2018. Until June 14, 2018, 510 cases were confirmed. According to the most recent bulletin released by the State Health Department on June 8, 2018, 441 occurrences are people residing in Santa Maria. Five are residents of the districts and seven cases are patients residing in neighboring counties. In a bulletin published on May 25, 2018, 1,116 cases were reported to state epidemiological surveillance by the end of May. Of these, 766 cases were still suspected (fever, headache and/or myalgia accompanied by lymphadenopathy, weakness, arthralgia, or change in vision. In the other 460 cases, there was laboratory confirmation of acute toxoplasmosis, of which 35 were pregnant, with two fetal deaths (36 and 28 weeks), and two abortions. There are also 212 cases still pending laboratory confirmation.Discussion:The results of this research show that the current outbreak of toxoplasmosis in the city of Santa Maria, Brazil, is the largest reported in Brazil and appears to be the largest in the world. The notification to authorities by physicians was very important for the identification of this outbreak.

Introduction:In the first months of 2018, there was an increase in the number of cases of fever possibly related to Toxoplasmosis in the city of Santa Maria, Brazil, reaching significant values. Toxoplasmosis is an autoimmune acute infection usually asymptomatic in 80-90% of immunocompetent adults. In this outbreak, attention is drawn to the intensity of the symptoms presented, which were observed in more than 70% of the cases.Objective: To report the cases of the outbreak of toxoplasmosis in the city of Santa Maria, Brazil.Methods: Cross-sectional study. Data were collected from bulletins published by the Municipal Health cases were confirmed. According to the most recent bulletin released by the State Health Department on June 8, 2018, 441 occurrences are people residing in Santa Maria, five are residents of the districts and seven cases are patients residing in neighboring counties. In a bulletin published on May 25, 2018, 1,116 cases were reported to state epidemiological surveillance by the end of May. Of these, 766 cases were still suspected (fever, headache and/or myalgia accompanied by lymphadenopathy, weakness, arthralgia or change in vision. In the other 460 cases, there was laboratory confirmation for acute toxoplasmosis, of which 35 were pregnant, with two fetal deaths (36 and 28 weeks) and two abortions. There are also 212 cases still pending laboratory confirmation, of which 133 were pregnant women, 1 was a spontaneously aborted fetus and 17 were children . Tests carried out on the water supplying the city so far have resulted negative for toxoplasmosis. Conclusion:The results of this research show that the current outbreak of toxoplasmosis in the city of Santa Maria, Brazil, is the largest reported in the world.