Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
La dependencia emocional se define como una necesidad afectiva extrema que una persona siente hacia su pareja. Se ha relacionado con el déficit de control de impulsos y con el estilo de apego preocupado. El presente estudio tiene como objetivos analizar la relación entre la dependencia emocional, el apego y la conducta impulsiva, así como, analizar si estas dos últimas predicen la dependencia emocional. Además, se analiza si la dependencia emocional media en la relación entre el apego y la impulsividad y, finalmente, se estudian las diferencias de género en las tres variables mencionadas. La muestra está formada por 1533 adolescentes (707 mujeres y 826 hombres), con edades que oscilan entre los 14-18 años (M = 15.76; DT = 1.25). Los resultados muestran relaciones positivas entre la dependencia emocional y la permisividad parental, traumatismo infantil e impulsividad. Asimismo, la impulsividad se relaciona de manera positiva con evitar estar solo, relación asimétrica, necesidad de agradar, permisividad parental y traumatismo infantil. Igualmente, se comprueba como la permisividad parental, autosuficiencia y rencor contra los padres y el traumatismo infantil predicen la dependencia emocional. Finalmente, se observa como la relación entre el apego y la conducta impulsiva es mediada por la dependencia emocional y se aprecian diferencias en las tres variables en función del género.
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