Ispinesib is a potent
inhibitor of kinesin spindle protein (KSP),
which has been identified as a promising target for antimitotic anticancer
drugs. Herein, we report the synthesis of half-sandwich complexes
of Ru, Os, Rh, and Ir bearing the ispinesib-derived
N
,
N
-bidentate ligands (
R
)- and (
S
)-2-(1-amino-2-methylpropyl)-3-benzyl-7-chloroquinazolin-4(3H)-one
and studies on their chemical and biological properties. Using the
enantiomerically pure (
R
)- and (
S
)-forms of the ligand, depending on the organometallic moiety, either
the
S
M
,
R
or
R
M
,
S
diastereomers, respectively, were
observed in the molecular structures of the Ru- and Os(cym) (cym =
η
6
-
p
-cymene) compounds, whereas
the
R
M
,
R
or
S
M
,
S
diastereomers were found for
the Rh- and Ir(Cp*) (Cp* = η
5
-pentamethylcyclopentadienyl)
derivatives. However, density functional theory (DFT) calculations
suggest that the energy difference between the diastereomers is very
small, and therefore a mixture of both will be present in solution.
The organometallics exhibited varying antiproliferative activity in
a series of human cancer cell lines, with the complexes featuring
the (
R
)-enantiomer of the ligand being more potent
than the (
S
)-configured counterparts. Notably, the
Rh and Ir complexes demonstrated high KSP inhibitory activity, even
at 1 nM concentration, which was independent of the chirality of the
ligand, whereas the Ru and especially the Os derivatives were much
less active.
The crystal structure of pyrene-1-carbaldehyde (PA), a model polyaromatic hydrocarbon, highly luminescent in the solid state and crystallizing in the triclinic system, has been re-determined at several pressures ranging from atmospheric up to 3 GPa using a diamond anvil cell. A 'multi-crystal' approach was used in crystal structure determination, significantly improving completeness of X-ray diffraction data attainable for such a low-symmetry system. The crystal structure consists of infinite -stacks of PA molecules with discernible dimers, which resemble aggregates formed by pyrene derivatives in solution as well as in the solid state. A series of measurements showed that the average inter-planar distance between individual molecules within -stacks decreases with pressure in the investigated range. This results in piezochromic properties of PA: a significant sample color change as well as a red-shift of fluorescence with pressure, as studied with UV-vis spectroscopy. Periodic DFT calculations allowed us to relate the variations in the crystal structure with pressure to the changes in the electronic structure of this material. research papers Acta Cryst. (2019). B75, 343-353 Tchoń and Makal Structure and piezochromism of pyrene-1-carbaldehyde 353
New polymorphs of 1,8-diacetylpyrene (2′′AP) exhibit distinct packing-dependent and pressure-dependent luminescence in the solid state and illustrate the usefulness of crystal structure prediction methods.
Sufficiently high completeness of diffraction data is necessary to correctly determine the space group, observe solid-state structural transformations or investigate charge density distribution under pressure. Regrettably, experiments performed at high pressure in a diamond anvil cell (DAC) yield inherently incomplete datasets. The present work systematizes the combined influence of radiation wavelength, DAC opening angle and sample orientation in a DAC on the completeness of diffraction data collected in a single-crystal high-pressure (HP) experiment with the help of dedicated software. In particular, the impact of the sample orientation on the achievable data completeness is quantified and proved to be substantial. Graphical guides for estimating the most beneficial sample orientation depending on the sample Laue class and assuming a few commonly used experimental setups are proposed. The usefulness of these guides has been tested in the case of luminescent 1,3-diacetylpyrene, suspected to undergo transitions from the α phase (Pnma) to the γ phase (Pn21
a) and δ phase (P1121/a) under pressure. Effective sample orientation has ensured over 90% coverage even for the monoclinic system and enabled unrestrained structure refinements and access to complete systematic extinction patterns.
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