Agricultural management systems are needed to simultaneously enhance production, promote plant diversity, improve nutrient cycling and reduce soil compaction. We investigated the effects of intercropped forage grass on production of corn (Zea mays L.) harvested for silage at 0.20 and 0.45 m height in the summer, as well as on production of subsequent forage, soybean [Glycine max (L.) Merr.] harvested for silage, nutrient cycling and soil responses on a Typic Haplorthox in Botucatu, São Paulo State, Brazil. Palisade grass cv. BRS Piatã [Urochloa brizantha cv. BRS Piatã] was the introduced companion crop with corn (Years 1 and 2), while signal grass [Urochloa decumbens cv. Basilisk] was the residual weedy species in comparison. Guineagrass cv. Aruãna [Megathyrsus maximus cv. Aruãna] was the introduced companion crop with soybean (Year 3), with only a residual effect of crop systems from the previous two years. After the corn silage harvest, pasture was grazed by lambs in winter/spring using a semi-feedlot system. When cut at 0.45 m compared with 0.20 m height, corn intercropped with palisade grass had greater leaf nutrient concentration, improved agronomic characteristics, forage mass of pasture for grazing by lambs, greater surface mulch produced, and greater quantity of N, P and K returned to soil. Greater soil organic matter, P, K and Mg concentration, and base saturation in the surface soil depth and lower soil penetration resistance at all depths occurred at 0.45 m than at 0.20 m corn silage cutting height intercropped with palisade grass. Analyzing the system as a whole, harvesting corn silage crop with palisade grass intercrop at 0.45 m height was the most viable option in this integrated crop-livestock system.

Agricultural management systems are needed to simultaneously enhance production, and improve soil quality. We investigated the effects of intercropped grass on production of corn (Zea mays L.) harvested for silage at 0.20 and 0.45 m height in the summer, as well as on production of subsequent forage, silage soybean [Glycine max (L.) Merr.], and soil responses on a Typic Haplorthox in Botucatu, SP, Brazil. Palisade grass [Urochloa brizantha (Hochst. ex A. Rich.) R. Webster ‘Marandu’] was the introduced companion crop with corn (Years 1 and 2), while signal grass [Urochloa decumbens (Stapf) R. Webster ‘Basilisk’] was the residual weedy species in comparison. Guinea grass [Urochloa maxima (Jacq.) R. Webster ‘Aruãna’] was the introduced companion crop with soybean (Year 3), with only a residual effect of crop systems from the previous 2 yr. When cut at 0.45 m compared with 0.20 m height, corn intercropped with palisade grass had greater leaf nutrient concentrations, agronomic characteristics, forage mass of pasture for grazing by lambs (Ovis aries), greater surface mulch produced, and greater quantity of N, P, and K returned to soil. Greater soil organic matter, P, K, and Mg concentration, and base saturation in the surface soil depth and lower soil penetration resistance at all depths occurred at 0.45 m than at 0.20 m corn silage cutting height intercropped with palisade grass. Analyzing the system as a whole, harvesting corn silage crop with palisade grass intercrop at 0.45 m height was the most viable option in this integrated crop–livestock system (ICLS).Core Ideas Agricultural management systems are needed to enhance production and improve soil quality. After corn silage harvest, pasture was grazed by lambs in winter/spring using a semi‐feedlot system. Harvesting corn silage crop with palisade grass intercrop at 0.45 m height was the most viable option.

Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.

-The objective of this work was to compare the yield and nutritive value of the silage of corn intercropped with palisade grass (Urochloa brizantha 'Marandu') or guinea grass (Megathyrsus maximus 'Tanzânia') with those of the silage of monocropped corn, as well as to evaluate the pasture established in the intercropping systems during two growing seasons (2010-2011 and 2011-2012), in low-altitude Brazilian Cerrado. The treatments consisted of three cropping systems: monocropped corn, corn intercropped with palisade grass, and corn intercropped with guinea grass, with four replicates. Intercropping decreased corn forage dry matter yield for silage; however, due to the addition of grass, total dry matter yield (corn + grass) was similar between treatments. Intercropping also did not negatively affect corn production components and morphological characteristics. The cropping systems provided silages with good nutritive values, and the inclusion of tropical forages increased the silage fiber contents. Intercropping corn with tropical perennial grasses is a viable option for producing large quantities of silage with good nutritive value. The forage yield (silage + pasture) of these intercropping systems is similar to that of monocropped corn in tropical regions and has the advantage of providing a pasture in the off-season.Index terms: Megathyrsus maximus, Urochloa brizantha, Zea mays, Brazilian Cerrado, intercropped system. Produtividade e valor nutritivo da silagem de milho em consórcio com gramíneas perenes tropicaisResumo -O objetivo deste trabalho foi comparar a produtividade e o valor nutritivo da silagem de milho em consórcio com capim-marandu (Urochloa brizantha 'Marandu') ou capim-tanzânia (Megathyrsus maximus 'Tanzânia') aos da silagem de milho em monocultivo, bem como avaliar a pastagem estabelecida nos sistemas consorciados durante duas estações de cultivo (2010-2011 e 2011-2012), em Cerrado brasileiro de baixa altitude. Os tratamentos consistiram de três sistemas de cultivo: milho em monocultivo, milho em consórcio com capim-marandu e milho em consórcio com capim-tanzânia, em quatro repetições. O cultivo consorciado diminuiu a produtividade de massa seca de forragem de milho para silagem; porém, devido à adição do capim, a produtividade total de massa seca (milho + capim) foi similar entre os tratamentos. O consórcio também não afetou negativamente os componentes de produção e as características morfológicas do milho. Os sistemas de cultivo produziram silagens com bons valores nutricionais, e a inclusão de forrageiras tropicais aumentou os teores de fibra na silagem. O consórcio do milho com gramíneas perenes tropicais é viável para a produção de grandes quantidades de silagem com boa qualidade nutricional. A produção de forragem (silagem + pastagem) desses sistemas consorciados é similar à do milho em monocultivo em regiões tropicais e tem a vantagem de proporcionar pastagem na entressafra.Termos para indexação: Megathyrsus maximus, Urochloa brizantha, Zea mays, Cerrado, sistema consorciado.

Sorghum [Sorghum bicolor (L.) Moench] intercropped with tropical grasses can be used in silage production systems to increase the dry matter yield per area, provide pasture after sorghum harvesting and improve land‐use efficiency (LUE). Our objective was to compare the production and quality of sorghum silage intercropped with palisade grass [Urochloa brizantha (A. Rich.) Stapf ‘Marandu’] or guinea grass [Megathyrsus maximus (Jacq.) B.K. Simon & S.W.L. Jacobs ‘Tanzânia’] with that of monocropped sorghum, the pasture in the off‐season, as well as the land equivalent ratios of these systems during two growing seasons (2010–2011 and 2011–2012). The experiment was designed as a randomized complete block with four replicates. The treatments were: monocropped sorghum silage, sorghum silage intercropped with palisade grass and sorghum silage intercropped with guinea grass. The productivity of the intercropped systems was as high as that of the monocropped sorghum. The development of the sorghum was not affected by intercropping with grasses. Intercropping sorghum with tropical grasses generally increases the fiber content of the silage compared with monocropped sorghum; however, this is a viable option for producing feed with adequate nutritional quality. Intercropping sorghum with palisade grass improved the land equivalent ratio, resulting in greater LUE. Intercropping sorghum with palisade and guinea grass also improved the relative N yield compared with monocropped sorghum and did not influence aggressivity and relative crowding coefficient. The main benefit of these intercropping systems is established grazing during the off‐season, which can be considered a good cultivation strategy for tropical regions.Core Ideas Intercropping crops in a no‐till system increase the diversification of agricultural production. Intercropping systems provide better uses of agricultural areas throughout the year. The intercropping systems productivity is as high as that of monocropped crops. Intercropping sorghum with grasses is a viable option for feed production. One of the intercropping systems benefits is established grazing during the off‐season.

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ResumoNeste trabalho, apresentamos reflexões acerca do ensino e aprendizagem de